Baculovirus Entry and Egress from Insect Cells
Baculovirus Entry and Egress from Insect Cells
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DOI：
10.1146/annurev-virology-092917-043356
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Theilmann, David A.
中科院分区：
文献类型：
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作者：
Blissard, Gary W.;Theilmann, David A.
杆状病毒是昆虫的大型DNA病毒，在许多宿主中具有高致病性。在感染循环中，杆状病毒产生两种类型的病毒体。这些病毒体表型在物理上和功能上是不同的，并且每一种在病毒的生物学中起关键作用。一种表型，即封闭衍生病毒（ODV），封闭在一种结晶蛋白内，有利于宿主的口腔感染。ODV包膜蛋白至少有9种，称为口服感染因子，是ODV感染昆虫中肠上皮细胞的重要因子。病毒通过出芽从中肠细胞排出，产生第二种病毒表型，即出芽病毒（BV）。BV在宿主昆虫的大多数其他组织中结合、进入和复制。BV的细胞识别和进入由单一的主要包膜糖蛋白介导：在一些杆状病毒中为GP 64，在另一些杆状病毒中为F。两种病毒体表型的进入和排出通过显著不同的机制发生，并反映了一个生命周期，其中ODV特别适合于口腔感染，而BV介导动物内感染的传播。
Baculoviruses are large DNA viruses of insects that are highly pathogenic in many hosts. In the infection cycle, baculoviruses produce two types of virions. These virion phenotypes are physically and functionally distinct, and each serves a critical role in the biology of the virus. One phenotype, the occlusion-derived virus (ODV), is occluded within a crystallized protein that facilitates oral infection of the host. A large complex of at least nine ODV envelope proteins called per os infectivity factors are critically important for ODV infection of insect midgut epithelial cells. Viral egress from midgut cells is by budding to produce a second virus phenotype, the budded virus (BV). BV binds, enters, and replicates in most other tissues of the host insect. Cell recognition and entry by BV are mediated by a single major envelope glycoprotein: GP64 in some baculoviruses and F in others. Entry and egress by the two virion phenotypes occur by dramatically different mechanisms and reflect a life cycle in which ODVis specifically adapted for oral infection while BV mediates dissemination of the infection within the animal.