FREQUENCY-DOMAIN ANALYSIS OF MEMBRANE CAPACITANCE OF CULTURED-CELLS (HELA AND MYELOMA) USING THE MICROPIPETTE TECHNIQUE
FREQUENCY-DOMAIN ANALYSIS OF MEMBRANE CAPACITANCE OF CULTURED-CELLS (HELA AND MYELOMA) USING THE MICROPIPETTE TECHNIQUE
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DOI：
10.1016/s0006-3495(90)82360-2
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发表时间：
1990-07-01
影响因子：
3.4
通讯作者：
TAKASHIMA, S
中科院分区：
文献类型：
--
作者：
ASAMI, K;TAKAHASHI, Y;TAKASHIMA, S
用微管方法研究了培养细胞(HeLa和小鼠骨髓瘤)的膜电容和电导。HeLa细胞和骨髓瘤细胞的膜容量平均值分别为1.9µF/cm2和1.0µF/cm2。这些数值与悬浮法测得的数值一致。悬浮法不能提供膜电导的信息，而微吸管法在泄漏电流可以忽略不计的情况下，即使是极小的膜电导也能测量。用这项技术发现，HeLa和骨髓瘤细胞的膜电导都是90-100µS/cm2。本研究的目的之一是建立膜电容的频率分布。然而，人们发现，在这项技术的分辨率范围内，这些细胞的膜电容与1赫兹到1千赫之间的频率无关。
The membrane capacitance and conductance of cultured cells (HeLa and mouse myeloma) are investigated using the micropipette method. Mean values of the membrane capacities were found to be 1.9 .mu.F/cm2 for HeLa cells and 1.0 .mu.F/cm2 for myeloma cells. These values are in agreement with those obtained using the suspension method. Whereas the suspension method is unable to provide the information on membrane conductance, the micropipette method is able to measure even an extremely small membrane conductance if leakage current is negligibly small. The membrane conductances were found, using this technique, to be .apprx.90-100 .mu.S/cm2 for both HeLa and myeloma cells. On of the purposes of this study is to establish the frequency profile of membrane capacitance. It was found, however, that membrane capacitances of these cells are independent of frequency between 1 Hz and 1 KHz within the resolution of this technique.