A sensor histidine kinase co-ordinates cell wall architecture with cell division in Bacillus subtilis
A sensor histidine kinase co-ordinates cell wall architecture with cell division in Bacillus subtilis
复制标题
DOI：
10.1111/j.1365-2958.2008.06308.x
复制
发表时间：
2008-08-01
影响因子：
3.6
通讯作者：
Hoch, James A.
中科院分区：
文献类型：
--
作者：
Fukushima, Tatsuya;Szurmant, Hendrik;Hoch, James A.
在快速生长的细菌中，驱动DNA合成、分裂隔膜形成和细胞壁合成和重塑的过程之间的协调一致的相互联系需要通过信号传导机制进行精确的协调，而这些机制在很大程度上是未知的。枯草芽孢杆菌的YycG（传感器组氨酸激酶）-YycF（响应调节因子/转录因子）双组分系统控制在细胞壁重塑和细胞分离中起作用的酶及其抑制剂的合成。在这里，它表明，YycG传感器组氨酸激酶是在生长细胞中的分裂隔膜的一个组成部分。在野生型和FtsZ缺失的无隔膜细胞中，类似于PO（4）调节的基因转录的YycF的RT-PCR定量表明YycG激酶对YycF的活性依赖于YycG定位于分裂隔膜。这些数据支持一个模型，其中YycG传感器激酶感知分裂隔膜处的信息，并调节自溶素和自溶素抑制剂的相互合成，以协调生长和分裂与细胞壁重组。
The concerted interconnection between processes driving DNA synthesis, division septum formation and cell wall synthesis and remodelling in rapidly growing bacteria requires precise co-ordination by signalling mechanisms that are, for the most part, unknown. The YycG (sensor histidine kinase)-YycF (response regulator/transcription factor) two-component system of Bacillus subtilis controls the synthesis of enzymes and their inhibitors that function in cell wall remodelling and cell separation. Here it is shown that the YycG sensor histidine kinase is a component of the division septum in growing cells. RT-PCR quantification of YycF similar to PO(4)-regulated gene transcription, in wild type and FtsZ-depleted, septum-less cells, indicated that YycG kinase activity on YycF is dependent on YycG localization to a division septum. The data support a model in which the YycG sensor kinase perceives information at the division septum and regulates the reciprocal synthesis of autolysins and autolysin inhibitors to co-ordinate growth and division with cell wall restructuring.