N-Acetyl-cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue
N-Acetyl-cysteine mediated inhibition of spermatogonial cells apoptosis against malathion exposure in testicular tissue
复制标题
DOI：
10.1002/jbt.22046
复制
发表时间：
2018-04-01
影响因子：
3.6
通讯作者：
Kumar, Vijay
中科院分区：
文献类型：
--
作者：
Bhardwaj, Jitender Kumar;Saraf, Priyanka;Kumar, Vijay
迄今为止的毒理学研究表明，过量使用马拉硫磷（一种有机磷杀虫剂）会对哺乳动物的生殖生理产生严重的不良影响。本研究的目的是评估马拉硫磷诱导的毒性在剂量和时间依赖性的方式与N-乙酰-L-半胱氨酸的缓解作用。使用MTT测定法监测睾丸生殖细胞活力，其中NAC是一种抗氧化剂，通过提高细胞活力的频率显著降低了马拉硫磷诱导的毒性。组织形态学分析表明，NAC成功地减少了马拉硫磷诱导的睾丸细胞的几个凋亡特征。EB/AO差异染色显示NAC补充后细胞凋亡百分比显著下降。NAC还减少了马拉硫磷诱导的DNA片段化，沿着氧化应激参数的显著降低，导致睾丸生殖细胞内脂质过氧化反应的降低和铁还原抗氧化能力的增强。因此，NAC减轻了马拉硫磷诱导的毒性，证明了其在不孕症治疗中的潜力。
Toxicological studies so far suggest that excessive use of malathion, an organophosphate insecticide, causes serious ill-effects in mammalian reproductive physiology. The present study aims at assessing malathion-induced toxicity in a dose- and time-dependent manner with mitigating effects of N-acetyl-L-cysteine. The testicular germ cell viability was monitored using MTT assay, where NAC, being an antioxidant significantly reduced malathion-induced toxicity by enhancing the frequency of cell viability. The histomorphological analysis showed that NAC successfully diminished several apoptotic features in testicular cells, induced by malathion. The differential EB/AO staining revealed a significant decline in the percentage of apoptosis after NAC supplementation. NAC also diminished the malathion-induced DNA fragmentation along with significantly reduction in oxidative stress parameters causing decrease in lipid peroxidation and enhancement of ferric reducing antioxidant power within testicular germ cells. Thus, NAC mitigated the malathion-induced toxicity, proving its potential in infertility treatment.