Candida albicans ALS3 and insights into the nature of the ALS gene family
Candida albicans ALS3 and insights into the nature of the ALS gene family
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DOI：
10.1007/s002940050359
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发表时间：
1998-06-01
期刊：
影响因子：
2.5
通讯作者：
Scherer, S
中科院分区：
文献类型：
--
作者：
Hoyer, LL;Payne, TL;Scherer, S
白色念珠菌的ALS 1（凝集素样序列）基因编码一种类似于α-凝集素的蛋白质，α-凝集素是酿酒酵母的一种细胞表面粘附糖蛋白（Hoyer et al. 1995）。一个串联重复的108-bp序列的中心域被发现在ALS 1编码区。该串联重复基序与多个C。提示在白色念珠菌中可能存在其他类似ALS 1的基因。白色念珠菌（Hoyer et al. 1995）。为了确定这些片段是否构成基因家族，使用基于ALS 1的共有串联重复序列的引物通过PCR筛选从fosmid文库中分离串联重复杂交基因组片段（Hoyer等，1995）。其中一组被命名为ALS 3的fossils编码一个与ALS 1具有81%同一性的基因。ALS 1和ALS 3的序列在串联重复区和串联重复区的5'端最为保守。ALS 3是菌丝特异性的。这两种基因都存在于多种C.白色念珠菌和念珠菌。检查了星状纲菌株。预测的Als 1 p和Als 3 p显示的功能表明，两者都是细胞表面的糖蛋白。用串联重复序列5'端的保守序列进行Southern印迹分析表明，在C.白念珠菌的基因组，ALS家族可能比最初估计的要大。
The ALS1 (agglutinin-like sequence) gene of Candida albicans encodes a protein similar to alpha-agglutinin, a cell-surface adhesion glycoprotein of Saccharomyces cerevisiae (Hoyer et al. 1995). A central domain of a tandemly repeated 108-bp sequence is found in the ALS1 coding region. This tandem-repeat motif hybridizes to multiple C. albicans genomic DNA fragments, indicating the possibility of other ALS1-like genes in C. albicans (Hoyer et al. 1995). To determine if these fragments constitute a gene family, tandem-repeat-hybridizing genomic fragments were isolated from a fosmid library by PCR screening using primers based on the consensus tandem-repeat sequence of ALS1 (Hoyer et al. 1995). One group of fosmids, designated ALS3, encodes a gene with 81% identity to ALS1. The sequences of ALS1 and ALS3 are most conserved in the tandem-repeat domain and in the region 5' of the tandem repeats, Northern-blot analysis using unique probes from the 3' end of each gene demonstrated that ALS1 expression varies, depending on which C. albicans strain is examined, and that,ALS3 is hyphal-specific. Both genes are found in a variety of C. albicans and C. stellatoidea strains examined. The predicted Als1p and Als3p exhibit features suggesting that both are cell-surface glycoproteins. Southern blots probed with conserved sequences from the region 5' of the tandem repeats suggest that other ALS-like sequences are present in the C. albicans genome and that the ALS family may be larger than originally estimated.