Implication of cyclooxygenase-2 on enhanced proliferation of neural progenitor cells in the adult mouse hippocampus after ischemia
Implication of cyclooxygenase-2 on enhanced proliferation of neural progenitor cells in the adult mouse hippocampus after ischemia
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DOI：
10.1002/jnr.10595
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发表时间：
2003-05-15
影响因子：
4.2
通讯作者：
Hori, M
中科院分区：
文献类型：
--
作者：
Sasaki, T;Kitagawa, K;Hori, M
全脑缺血促进成年小鼠海马齿状回神经发生。环氧合酶（考克斯）-2是脑中的主要同工酶，调节炎症、谷氨酸介导的细胞毒性和突触可塑性。我们证明，延迟治疗与不同类别的考克斯抑制剂显着钝化增强缺血后齿状回神经前体细胞的增殖。考克斯-2的免疫反应，观察到神经元和星形胶质细胞在齿状回，但不是在神经前体细胞在颗粒下区。此外，在杂合和纯合考克斯-2基因敲除小鼠的缺血后齿状回中，增殖的溴脱氧尿苷阳性细胞明显少于野生型同窝小鼠。这些结果表明，考克斯-2是促进缺血后神经前体细胞增殖的重要调节剂。(C)2003 Wiley-Liss，Inc.
Global ischemia promotes neurogenesis in the dentate gyrus of the adult mouse hippocampus. Cyclooxygenase (COX)-2, the principal isoenzyme in the brain, modulates inflammation, glutamate-mediated cytotoxicity, and synaptic plasticity. We demonstrated that delayed treatment with different classes of COX inhibitor significantly blunted enhancement of dentate gyrus proliferation of neural progenitor cells after ischemia. COX-2 immunoreactivity was observed in both neurons and astrocytes in the dentate gyrus, but not in neural progenitor cells in the subgranular zone. Moreover, in the postischemic dentate gyrus of heterozygous and homozygous COX-2 knockout mice, proliferating bromodeoxyuridine-positive cells were significantly fewer than in wild-type littermates. These results demonstrate that COX-2 is an important modulator in enhancement of proliferation of neural progenitor cells after ischemia. (C) 2003 Wiley-Liss, inc.