Efficient incorporation of quantum dots into porous microspheres through a solvent-evaporation approach.
Efficient incorporation of quantum dots into porous microspheres through a solvent-evaporation approach.
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DOI：
10.1021/la300066c
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Gang Wang;Pengfei Zhang;Hongjing Dou;Wanwan Li;K. Sun;Xiao-Yue He;Junsong Han;Hua-sheng Xiao;Y. Li
中科院分区：
文献类型：
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作者：
Gang Wang;Pengfei Zhang;Hongjing Dou;Wanwan Li;K. Sun;Xiao-Yue He;Junsong Han;Hua-sheng Xiao;Y. Li
量子点编码微球作为生物分子间各种反应的载体，在悬浮液阵列中发挥着重要的作用。对于用于悬浮阵列的量子点编码微球，三个关键要求是尺寸可控，表面官能团丰富，尤其是优异的荧光性能。采用种子共聚法制备了具有特定粒径、表面羧基和多孔结构的窄分散聚苯乙烯-共二乙烯基苯-共甲基丙烯酸（PSDM）微球。为了提高量子点在微球内的掺入效率，我们开发了一种膨胀-蒸发方法，其中膨胀过程与溶剂的逐渐蒸发相结合，从而使量子点在分散溶液中逐渐集中。这种方法被证明是一种有效的方法，以提高所得微球的荧光强度与单独使用膨胀。此外，多孔结构有助于量子点渗透到微球内部。通过这种方法，可以有效地制备出由单个或多个波长发射量子点编码的微球。利用量子点编码微球，包被小鼠抗人绒毛膜促性腺激素作为检测山羊抗小鼠IgG的探针，建立悬浮免疫检测方法。Luminex 100检测的阳性结果和qd编码微球的低细胞毒性显示了它们在悬浮阵列中的巨大潜力。
Quantum dot (QD)-encoded microspheres play an important role in suspension arrays by acting as supports for various reactions between biomolecules. With regard to QD-encoded microspheres utilized in suspension arrays, three key requirements are controllable size, abundant surface functional groups, and especially excellent fluorescence properties. In this paper, narrowly dispersed poly(styrene-co-divinylbenzene-co-methylacrylic acid) (PSDM) microspheres with specific size, surface carboxyl groups, and porous structures were synthesized by seeded copolymerization. In order to improve the incorporation efficiency of QDs within microspheres, we developed a swelling-evaporation approach in which the swelling process was combined with gradual evaporation of the solvent and thus gradual concentration of QDs in the dispersion solution. This approach was demonstrated to be an efficient method for improving the fluorescence intensity of resultant microspheres compared with the use of swelling alone. Moreover, the porous structure was shown to aid the penetration of QDs into the interiors of the microspheres. Through this approach, microspheres encoded with either single or multiple wavelength-emitting QDs were fabricated effectively. The suspension immunoassays were then founded based on the QD-encoded microspheres, by coating mouse antihuman chorionic gonadotropin as the probe for goat antimouse IgG detection. The positive results determined by Luminex 100 and the low cytotoxicity of the QD-encoded microspheres demonstrated their great potential in suspension arrays.