Location and characterization of female germline stem cells (FGSCs) in juvenile porcine ovary
Location and characterization of female germline stem cells (FGSCs) in juvenile porcine ovary
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DOI：
10.1111/cpr.12058
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发表时间：
2013-10-01
影响因子：
8.5
通讯作者：
Hua, J.
中科院分区：
文献类型：
--
作者：
Bai, Y.;Yu, M.;Hua, J.
目的自从有报道称成年小鼠卵巢中仍存在有丝分裂活性的生殖细胞以来，幼年哺乳动物雌性卵巢中生殖干细胞的存在一直是一个争论不休的话题。此外，明确的位置，这样的女性生殖系干细胞（FGSCs）还没有demonstrated.Materials和methodsWe分离猪FGSCs从卵巢皮质机械，并测试我们的假设，利用免疫荧光染色，qRT-PCR和westernblotting.ResultsWe重视明确的位置FGSCs，同时解决在卵泡膜。体外培养4个月以上，猪卵泡膜层细胞与小鼠FGSCs和ESCs具有相似的特性。结论这些结果为研究卵子发生和治疗卵巢不育症提供了新的来源。
ObjectivesExistence of germline stem cells (GSCs) in juvenile mammalian female ovaries has been drastically debated recently since reports that adult mouse ovaries still have mitotically active germ cells have been proposed. In addition, definitive location of such female germline stem cells (FGSCs) had not been demonstrated.Materials and methodsWe segregated porcine FGSCs mechanically from ovary cortex, and tested our hypotheses by utilizing immunofluorescent staining, qRT-PCR and western blotting.ResultsWe attached emphasis to unambiguous location of FGSCs, which settle simultaneously in the theca. Dissected cells from porcine thecal layers maintained similar characteristics to mouse FGSCs and ESCs over 4-months in vitro culture.ConclusionThese results may provide a new resource for the study of oogenesis and therapy for ovarian sterility.