The Matrix Gene Expression of Subacute Sclerosing Panencephalitis (SSPE) Virus (Osaka‐1 Strain): A Comparison of Two Sibling Viruses Isolated from Different Lobes of an SSPE Brain
The Matrix Gene Expression of Subacute Sclerosing Panencephalitis (SSPE) Virus (Osaka‐1 Strain): A Comparison of Two Sibling Viruses Isolated from Different Lobes of an SSPE Brain
复制标题
亚急性硬化性全脑炎 (SSPE) 病毒（Osaka-1 株）的基质基因表达：从 SSPE 脑不同叶分离的两种兄弟病毒的比较
DOI：
10.1111/j.1348-0421.1998.tb02351.x
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发表时间：
1998
影响因子：
2.6
通讯作者：
H. Ogura
中科院分区：
文献类型：
--
作者：
M. Ayata;K. Hayashi;T. Seto;R. Murata;H. Ogura
The Fr/V and Oc/V sibling viruses of the Osaka‐1 strain of the subacute sclerosing panencephalitis (SSPE) virus were defective in cell‐free virus production. By radioimmunoprecipitation assay, the matrix (M) protein was not detected in cells persistently infected with the Osaka‐1 strain. This undetectable expression was consistent with the selective reduction of antibody response to the M protein in the patient from whom the Osaka‐1 strain was isolated. The sequence of the M gene, however, predicted that the protein could be synthesized because the translational start and stop codons for the protein were not altered. Northern blot hybridization demonstrated the selective defect of the monocistronic mRNAs for the M protein and the phosphoprotein (P) together with the dominant presence of the P‐M bicistronic mRNA. This absence of the M mRNA was further confirmed by primer extension analysis. Therefore, the undetectable expression of the M protein in the infected cells was proved to be caused by a transcriptional defect. The two sibling viruses, isolated from remote portions of an SSPE brain, were indistinguishable in their viral characters, including the M gene sequences, which indicates the possibility of clonal expansion of the strain in the brain.
