Molecular Cytogenetic Analysis and Isolation of a 5S rRNA-Related Marker in the Scleractinian Coral Platygyra contorta Veron 1990 (Hexacorallia, Anthozoa, Cnidaria)
Molecular Cytogenetic Analysis and Isolation of a 5S rRNA-Related Marker in the Scleractinian Coral Platygyra contorta Veron 1990 (Hexacorallia, Anthozoa, Cnidaria)
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DOI：
10.1508/cytologia.82.205
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发表时间：
2017-03-01
期刊：
影响因子：
1
通讯作者：
Tominaga, Akira
中科院分区：
文献类型：
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作者：
Taguchi, Takahiro;Kubota, Satoshi;Tominaga, Akira
对在日本沿着温带海岸常见的石珊瑚Platygyra contorta进行了分子细胞遗传学分析。通过常规的G-和C-显带对捻转扁虫进行核型分析（2n=28），核型图显示约50%的中期铺展在12号染色体长臂的末端部分具有均匀染色区域（hsr）。荧光原位杂交（FISH）结果表明，该hsr由C-显带的rRNA基因（rDNA）组成。一个hsr的存在，这是一个高度扩增的rDNA，可以解释珊瑚rDNA的分子多样性。使用人类端粒探针，FISH显示并证明了该珊瑚中存在端粒基序（TTAGGG）（n）。此外，我们还分离到一个特异的FISH标记（312 bp），命名为PC-T1，它位于11号染色体的着丝粒附近。序列分析表明，PC-T1的末端（距3'端51 bp）与Montipora capitate微卫星和Actinia equine 5SrRNA基因有90%的同源性。FISH标记的分离将有助于描述其基因组的进展，石珊瑚的分类。本研究所获得的数据对于区分石珊瑚的种类和了解它们的遗传学，包括染色体进化，将是有价值的。
A molecular cytogenetic analysis was conducted on the scleractinian coral Platygyra contorta, which is commonly found along temperate coasts in Japan. P. contorta was karyotyped (2n=28) by conventional G- and C-bandings, and the karyogram revealed that about 50% of the metaphase spreads had a homogenously staining region (hsr) in the terminal portion of the long arm of chromosome 12. Fluorescence in situ hybridization (FISH) showed that this hsr consisted of rRNA genes (rDNA) stained by C-banding. The presence of an hsr, which is a highly amplified rDNA, may explain the molecular diversity of coral rDNA. FISH visualized and demonstrated the presence of a telomere motif (TTAGGG)(n) in this coral using a human telomere probe. Furthermore, we isolated a specific FISH marker (312 bp) designated PC-T1, which was located near the centromere of chromosome 11. A sequence analysis revealed that the terminal part of PC-T1 (51 bp from the 3' end) was 90% homologous with a Montipora capitate microsatellite and the Actinia equine 5S rRNA gene. Isolating FISH markers will assist the classification of scleractinian corals with the progression on describing their genome. The data obtained in this study will be valuable for discriminating species among scleractinian corals and understanding their genetics, including chromosomal evolution.