Large organellar changes occur during mild heat shock in yeast.
Large organellar changes occur during mild heat shock in yeast.
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DOI：
10.1242/jcs.258325
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发表时间：
2022-03-01
影响因子：
4
通讯作者：
Höög JL
中科院分区：
文献类型：
--
作者：
Keuenhof KS;Larsson Berglund L;Malmgren Hill S;Schneider KL;Widlund PO;Nyström T;Höög JL
当温度升高时，热休克反应被激活以保护细胞环境。这一过程的转录组学和蛋白质组学得到了深入的研究，而关于细胞如何在结构上对热应激做出反应的信息大多缺乏。在此，使酿酒酵母经受温和的连续热冲击（38°C）并间歇地冷冻固定用于电子显微镜检查。通过测量2100多张电子显微照片中所有可区分的细胞器数量，大小和形态的变化，揭示了渐进式热休克期间细胞内部结构的重大重组。细胞变大了，但其中的大多数细胞器膨胀得更多，使细胞质体积缩小。细胞器在不同的时间，无论是在大小和数量方面的热休克，和适应的一些细胞器（如液泡）的形态进行了观察。多泡体增长了近70%，表明以前未知的参与热休克反应。一种以前未描述的电子半透明结构聚集在质膜附近。这种包罗万象的方法提供了一个详细的时间顺序的细胞器适应整个细胞热应激反应。总结：暴露于轻度热休克导致酵母细胞超微结构的大的可量化的变化，显示多泡体参与热休克反应和新结构的出现。
When the temperature is increased, the heat-shock response is activated to protect the cellular environment. The transcriptomics and proteomics of this process are intensively studied, while information about how the cell responds structurally to heat stress is mostly lacking. Here, Saccharomyces cerevisiae were subjected to a mild continuous heat shock (38°C) and intermittently cryo-immobilised for electron microscopy. Through measuring changes in all distinguishable organelle numbers, sizes and morphologies in over 2100 electron micrographs, a major restructuring of the internal architecture of the cell during the progressive heat shock was revealed. The cell grew larger but most organelles within it expanded even more, shrinking the volume of the cytoplasm. Organelles responded to heat shock at different times, both in terms of size and number, and adaptations of the morphology of some organelles (such as the vacuole) were observed. Multivesicular bodies grew by almost 70%, indicating a previously unknown involvement in the heat-shock response. A previously undescribed electron-translucent structure accumulated close to the plasma membrane. This all-encompassing approach provides a detailed chronological progression of organelle adaptation throughout the cellular heat-stress response. Summary: Exposure to mild heat shock leads to large quantifiable changes in the cellular ultrastructure of yeast, showing the involvement of multivesicular bodies in the heat-shock response and the apparition of novel structures.