Accumulation of Hsp70 mRNA under environmental stresses in diapausing and nondiapausing adults of Drosophila triauraria
Accumulation of Hsp70 mRNA under environmental stresses in diapausing and nondiapausing adults of Drosophila triauraria
复制标题
DOI：
10.1016/s0022-1910(97)00143-1
复制
发表时间：
1998-10-01
影响因子：
2.2
通讯作者：
Kimura, MT
中科院分区：
文献类型：
--
作者：
Goto, SG;Yoshida, KM;Kimura, MT
果蝇进入生殖滞育响应短日照和获得耐受热，冷和干燥。在这个物种中，热休克反应（热休克蛋白70 mRNA的积累，以响应热）发生在27-41摄氏度，和Hsp 70 mRNA的水平之间没有差异滞育和nondiapausing个人。热休克蛋白70的mRNA也被诱导暴露于-4或-8 ℃。然而，在暴露于冷后几乎没有检测到，而是在暴露于冷后保持在常温下时积累。在-4 ℃时，滞育个体的Hsp 70 mRNA水平低于非滞育个体，而在-8 ℃时，两者之间没有差异。无论滞育状态如何，在干燥胁迫下，该物种都不合成Hsp 70 mRNA。这些结果表明，滞育个体获得的热，冷和干燥的耐受性，独立的热休克蛋白70基因的转录调控。(C)1998爱思唯尔科技有限公司版权所有。
Drosophila triauraria entered reproductive diapause in response to short daylengths and acquired tolerance to heat, cold and desiccation. In this species, the heat-shock response (accumulation of Hsp70 mRNA in response to heat) occurred at 27-41 degrees C, and the level of Hsp70 mRNA did not differ between diapausing and nondiapausing individuals. Hsp70 mRNA was also induced by exposure to -4 or -8 degrees C. However, it was scarcely detected just after the exposure to cold, but accumulated when flies were maintained at normal temperature following the exposure to cold. The level of Hsp70 mRNA was lower in diapausing individuals than in nondiapausing ones when exposed to -4 degrees C, but was not different between them when exposed to -8 degrees C. This species did not synthesize Hsp70 mRNA under desiccation stress irrespective of the diapause state. These results suggest that diapausing individuals of this species acquired tolerance to heat, cold and desiccation independent of the transcriptional regulation of the hsp70 gene. (C) 1998 Elsevier Science Ltd. All rights reserved.