Mice refractory to lipopolysaccharide manifest high immunoglobulin A responses to orally administered antigen.
Mice refractory to lipopolysaccharide manifest high immunoglobulin A responses to orally administered antigen.
复制标题
对脂多糖耐药的小鼠表现出对口服抗原的高免疫球蛋白 A 反应。
DOI：
10.1128/iai.29.2.322-328.1980
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发表时间：
1980
影响因子：
3.1
通讯作者：
McGhee,JR
中科院分区：
文献类型：
--
作者：
Babb,JL;McGhee,JR
与同基因脂质 A 反应的 C3H/HeN 小鼠相比，脂质 A 无反应的 C3H/HeJ 小鼠对口服（通过喂养或胃内免疫）异源红细胞表现出高免疫反应。长时间消耗马红细胞会导致两种 C3H 小鼠品系产生显着的分泌性免疫反应，如高唾液凝集素滴度所证明的那样。尽管 C3H/HeJ 小鼠的唾液凝集素滴度仅略高于 C3H/HeN 小鼠，但 C3H/HeJ 小鼠的血清凝集素滴度和免疫球蛋白 A (IgA) 水平始终较高（2 至 4 倍）。在免疫动物的脾脏中出现抗马红细胞噬斑形成细胞反应后，唾液抗马红细胞凝集素活性增加。免疫接种约 3 周后，两种反应均达到峰值水平。 C3H 小鼠品系之间的免疫反应差异在细胞水平上也很明显，因为喂食的 C3H/HeJ 小鼠中脾 IgA 抗马红细胞斑形成细胞反应比接受类似处理的 C3H/HeN 小鼠高两倍。当 C3H/HeJ 小鼠对胃内施用的绵羊红细胞表现出三倍高的脾 IgM 和 IgA 空斑形成细胞反应时，也观察到这种较高的反应模式。因此，在通过口服途径给予异源红细胞的 C3H/HeJ 小鼠中观察到更高的反应性。此外，10至12个月大的未免疫C3H/HeJ小鼠的血清IgA水平高于C3H/HeN小鼠。这些发现表明，宿主对脂多糖缺乏反应性会影响随后对口服抗原的免疫反应的表现。讨论了这种高响应性的可能机制和影响。
Lipid A-nonresponding C3H/HeJ mice manifested high immune responses to orally administered (either by feeding or by intragastric immunization) heterologous erythrocytes when compared with syngeneic lipid A-responding C3H/HeN mice. Prolonged consumption of horse erythrocytes resulted in a significant secretory immune response in both C3H mouse strains as evidenced by high salivary agglutinin titers. Although salivary agglutinin titers were only slightly greater in C3H/HeJ mice than those of C3H/HeN mice, serum agglutinin titers and immunoglobulin A (IgA) levels were consistently higher (two- to fourfold) in C3H/HeJ mice. The appearance of anti-horse erythrocyte plaque-forming cell responses in spleens of immunized animals was followed by an increase in salivary anti-horse erythrocyte agglutinin activity. Peak levels of both responses were attained after approximately 3 weeks of immunization. Differences in immune responsiveness between C3H mouse strains were also evident at the cellular level since splenic IgA anti-horse erythrocyte plaque-forming cell responses in fed C3H/HeJ mice were twofold higher than those in similarly treated C3H/HeN mice. This higher response pattern was also observed when C3H/HeJ mice manifested threefold higher splenic IgM and IgA plaque-forming cell responses to intragastrically administered sheep erythrocytes. Thus, higher responsiveness was observed in the C3H/HeJ mice given heterologous erythrocytes by the oral route. Furthermore, levels of serum IgA in 10- to 12-month-old nonimmunized C3H/HeJ mice were higher than those of C3H/HeN mice. These findings suggest that lack of host responsiveness to lipopolysaccharide affects the manifestation of subsequent immune responses to orally administered antigens. The possible mechanisms and implications of this high responsiveness are discussed.