Comparison of the proteins of two immunologically distinct intermediate-sized filaments by amino acid sequence analysis: desmin and vimentin.
Comparison of the proteins of two immunologically distinct intermediate-sized filaments by amino acid sequence analysis: desmin and vimentin.
复制标题
通过氨基酸序列分析比较两种免疫学上不同的中等大小丝的蛋白质：结蛋白和波形蛋白。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
K. Weber
中科院分区：
文献类型：
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作者：
N. Geisler;K. Weber
虽然所有的中等大小的细丝（10纳米的细丝）似乎表现出相似的形态和共享一些生化特性，不同的细胞和组织特异性的亚类已区分免疫学实验和表观分子量和等电点的主要组成蛋白质的差异。为了了解这些蛋白质之间可能的同源性程度，我们已经开始对多肽进行氨基酸序列分析。在这里，我们的特点是一个大片段的鸡砂囊和猪胃结蛋白以及相应的片段从猪眼透镜波形蛋白。片段位于羧基末端，由138-140个氨基酸残基组成，即，约28%的相应多肽链。结果表明，这两种免疫学上不同的猪蛋白是不同的基因产物。它们显示了相关的氨基酸序列，但在羧基末端区域存在36%的残基。因此，组织特异性压倒物种分歧。这些结果进行了讨论，根据以前的免疫学实验。它们进一步支持了中间丝属于多基因家族的假设，该家族在胚胎发生期间按照一定的分化规则表达。
Although all intermediate-size filaments (10-nm filaments) seem to show similar morphology and share a number of biochemical properties, different cell- and tissue-specific subclasses have been distinguished by immunological experiments and by differences in apparent molecular weights and isoelectric points of the major constituent proteins. In order to understand the degree of possible homology between these proteins, we have begun amino acid sequence analysis of the polypeptides. Here we characterize a large fragment of chicken gizzard and pig stomach desmin as well as the corresponding fragment from porcine eye lens vimentin. The fragments are situated at the carboxyl end and consist of 138-140 amino acid residues--i.e., some 28% of the corresponding polypeptide chains. The results show that the two immunologically distinct porcine proteins are different gene products. They show a related amino acid sequence but differ in 36% of the residues present in the carboxy-terminal region. Thus tissue specificity overrides species divergence. These results are discussed in the light of previous immunological experiments. They lend further support to the hypothesis that intermediate filaments belong to a multigene family, which is expressed in line with certain rules of differentiation during embryogenesis.