Agrobacterium rhizogenes transformed calli of the holoparasitic plant Phelipanche ramosa maintain parasitic competence
Agrobacterium rhizogenes transformed calli of the holoparasitic plant Phelipanche ramosa maintain parasitic competence
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DOI：
10.1007/s11240-018-1466-x
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发表时间：
2018-08
期刊：
影响因子：
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通讯作者：
Dagmara Libiaková;C. Ruyter-Spira;H. Bouwmeester;R. Matusova
中科院分区：
文献类型：
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作者：
Dagmara Libiaková;C. Ruyter-Spira;H. Bouwmeester;R. Matusova
菲力班奇和奥罗班切斯。(高粱)是重要的经济寄生杂草，对许多农作物造成严重危害。然而，控制这些寄生杂草的常规方法往往并不有效。针对在寄生虫和宿主之间建立联系所涉及的分子和生化过程可能为控制提供一个新的视角。然而，由于这些寄生虫特定的生命周期，其遗传转化和再生即使不是不可能，也是困难的，这一事实阻碍了对这些过程的理解。是需要附着在寄主植物的根部才能使它们的同化物、营养物质和水分发育和繁殖的全寄生虫。本研究描述了一种高效的根寄生黄连的遗传转化和再生方案。我们提出了一种新的P的转换系统。发根农杆菌MSU440携带编码红色荧光蛋白(DsRed1)的非破坏性选择标记基因。利用该方法，转化效率可达90%。我们改造了4周大的P。然后将表达DsRed1的转基因愈伤组织培养在寄主植物上。首次报道了转基因寄生植物P。在转基因愈伤组织与寄主植物的根成功连接后，获得了分株。此外，我们还首次介绍了OFP的增长和发展。在没有寄主植物的情况下进行试管苗和花的培养。
PhelipancheandOrobanchespp. (broomrapes) are economically important parasitic weeds, causing severe damage to many agricultural crops. However, conventional methods to control these parasitic weeds are often not effective. Targeting molecular and biochemical processes involved in the establishment of the connection between the parasite and the host may offer a new perspective for control. However, progress in the understanding of these processes is hampered by the fact that genetic transformation and regeneration of these parasites is difficult if not impossible due to their specific lifecycle.PhelipancheandOrobanchespp. are holoparasites that need to attach to the roots of a host plant to get their assimilates, nutrients and water to develop and reproduce. The present study describes a highly efficient genetic transformation and regeneration protocol for the root holoparasiticPhelipanche ramosa. We present a new transformation system forP. ramosausingAgrobacterium rhizogenes MSU440carrying a non-destructive selection marker gene coding for a red fluorescent protein (DsRed1). Using this protocol up to 90% transformation efficiency was obtained. We transformed 4 weeks oldP. ramosacalli and transgenic calli expressing DsRed1 were then cultured on host plants. For the first time, we present shoot and flower development of the transgenic parasitic plantP. ramosaafter successful connection of transgenic calli with the host plant roots. Moreover, we also present, for the first time, growth and development ofP. ramosashoots and flowers in vitro in the absence of a host plant.