PLASTICITY OF THE DIFFERENTIATED STATE
PLASTICITY OF THE DIFFERENTIATED STATE
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DOI：
10.1126/science.2414846
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
WEBSTER, C
中科院分区：
文献类型：
--
作者：
BLAU, HM;PAVLATH, GK;WEBSTER, C
异核体提供了一个模型系统，用于研究组织特异性表型是如何产生和维持的。当肌肉细胞与非肌肉细胞融合时，非肌肉细胞类型的肌肉基因表达被激活。用单抗在单细胞水平上研究基因表达，或在生物化学和分子水平上在大规模培养中研究基因表达。在所有被测试的非肌肉细胞类型中，包括不同胚胎谱系、表型和发育阶段的代表，肌肉基因表达被诱导。不同类型的细胞对基因表达的动力学、频率和基因剂量要求的差异为潜在的调控机制提供了线索。这些结果表明，分化细胞的细胞核中的基因表达具有明显的可塑性，并且容易受到细胞质的调节。分离编码负责肌肉基因激活的组织特异性反式作用调节因子的基因现在应该是可能的。
Heterokaryons provide a model system in which to examine how tissue-specific phenotypes arise and are maintained. When muscle cells are fused with nonmuscle cells, muscle gene expression is activated in the nonmuscle cell type. Gene expression was studied either at a single cell level with monoclonal antibodies or in mass cultures at a biochemical and molecular level. In all of the nonmuscle cell types tested, including representatives of different embryonic lineages, phenotypes, and developmental stages, muscle gene expression was induced. Differences among cell types in the kinetics, frequency, and gene dosage requirements for gene expression provide clues to the underlying regulatory mechanisms. These results show that the expression of genes in the nuclei of differentiated cells is remarkably plastic and susceptible to modulation by the cytoplasm. The isolation of the genes encoding the tissue-specifictrans-acting regulators responsible for muscle gene activation should now be possible.