Maturation State-Dependent Alterations in Meniscus Integration: Implications for Scaffold Design and Tissue Engineering
Maturation State-Dependent Alterations in Meniscus Integration: Implications for Scaffold Design and Tissue Engineering
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DOI：
10.1089/ten.tea.2010.0272
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发表时间：
2011-01-01
影响因子：
4.1
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Ionescu, Lara C.;Lee, Gregory C.;Mauck, Robert L.
膝关节半月板是膝关节的重要组成部分，其功能是稳定关节、分配负荷和保持一致性。半月板撕裂和退化是常见的，自然愈合是有限的。值得注意的是，在未成熟的动物和胎儿中，很少有半月板损伤和其他相关纤维软骨组织再生愈合的儿童。在这项工作中，我们评估了胎儿，青少年和成年牛半月板的性能和修复能力在体外。尽管随着年龄的增长，细胞行为（迁移和增殖）没有变化，但半月板组织主要成分（蛋白多糖、胶原和DNA）的密度和分布随着发育发生了剧烈变化。与这些明显的组织变化同时，组织的体外愈合能力随着年龄的增长而下降。胎儿和青少年半月板形成了一个强大的修复超过8周的组织学和机械的基础上，尽管缺乏血管供应。相比之下，成人半月板在此期间没有整合。然而，随着生长因子转化生长因子-β 3的加入，整合显著改善。最后，为了评估工程支架在老化背景下的整合，我们监测了从天然组织到工程纳米纤维结构中的细胞浸润。我们的研究结果表明，成熟的过程，使负载在成人限制内源性愈合潜力，并确定组织工程半月板植入物的发展的新指标。
The knee meniscus is a crucial component of the knee that functions to stabilize the joint, distribute load, and maintain congruency. Meniscus tears and degeneration are common, and natural healing is limited. Notably, few children present with meniscus injuries and other related fibrocartilaginous tissues heal regeneratively in immature animals and in the fetus. In this work, we evaluated fetal, juvenile, and adult bovine meniscus properties and repair capacity in vitro. Although no changes in cell behavior (migration and proliferation) were noted with age, drastic alterations in the density and distribution of the major components of meniscus tissue (proteoglycan, collagen, and DNA) occurred with development. Coincident with these marked tissue changes, the in vitro healing capacity of the tissue decreased with age. Fetal and juvenile meniscus formed a robust repair over 8 weeks on both a histological and mechanical basis, despite a lack of vascular supply. In contrast, adult meniscus did not integrate over this period. However, integration was improved significantly with the addition of the growth factor transforming growth factor-beta 3. Finally, to evaluate engineered scaffold integration in the context of aging, we monitored cellular infiltration from native tissue into engineered nanofibrous constructs. Our findings suggest that maturation processes that enable load bearing in the adult limit endogenous healing potential and identify new metrics for the development of tissue-engineered meniscus implants.