SPECIFIC STIMULATION OF ACTIN GENE-TRANSCRIPTION BY EPIDERMAL GROWTH-FACTOR AND CYCLOHEXIMIDE
SPECIFIC STIMULATION OF ACTIN GENE-TRANSCRIPTION BY EPIDERMAL GROWTH-FACTOR AND CYCLOHEXIMIDE
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DOI：
10.1073/pnas.81.23.7476
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
GETZ, MJ
中科院分区：
文献类型：
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作者：
ELDER, PK;SCHMIDT, LJ;GETZ, MJ
用表皮生长因子（EGF）刺激静止的AKR-2B小鼠胚胎细胞导致肌动蛋白mRNA序列的快速和特异性诱导。这些mRNA包括编码β-和γ-细胞骨架，但不是α-骨骼肌肌动蛋白同种型在分离的细胞核中新生RNA链的延长（径流转录）表明，mRNA的积累之前，肌动蛋白基因转录的增加。然而，这种增加是瞬时的，并且随后是转录活性的快速衰减。蛋白质合成的抑制剂放线菌酮也诱导β-和γ-肌动蛋白序列在稳态核和多核糖体RNA。径流转录实验表明，这种协同效应的结果从肌动蛋白基因转录的幅度和持续时间的增加。它也是特定的，因为α-微管蛋白基因转录没有受到类似的影响。这些数据表明，存在一个特定的不稳定的肌动蛋白基因转录阻遏物。
Stimulation of quiescent AKR-2B mouse embryo cells with epidermal growth factor (EGF) results in a rapid and specific induction of actin mRNA sequences. These mRNA include those coding for both .beta.- and .gamma.-cytoskeletal, but not .alpha.-skeletal muscle, actin isotypes. Elongation of nascent RNA chains in isolated nuclei (run-off transcription) demonstrates that the mRNA accumulation is preceded by an increase in actin gene transcription. This increase is transient, however, and is followed by a rapid attenuation of transcriptional activity. An inhibitor of protein synthesis, cycloheximide, also induced .beta.- and .gamma.-actin sequences in both steady-state nuclear and polysomal RNA. Run-off transcription experiments demonstrate that this synergistic effect results from an increase in the magnitude and duration of actin gene transcription. It is also specific in that .alpha.-tubulin gene transcription is not similarly affected. These data suggest the existence of a specific labile repressor of actin gene transcription.