Antioxidative protection and proteolytic activity in tolerant and sensitive wheat (Triticum aestivum L.) varieties subjected to long-term field drought
Antioxidative protection and proteolytic activity in tolerant and sensitive wheat (Triticum aestivum L.) varieties subjected to long-term field drought
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DOI：
10.1007/s10725-008-9356-6
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发表时间：
2009-05-01
影响因子：
4.2
通讯作者：
Feller, Urs
中科院分区：
文献类型：
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作者：
Simova-Stoilova, Lyudmila;Demirevska, Klimentina;Feller, Urs
进行了田间干旱研究，以评估自然胁迫条件下的氧化胁迫、蛋白水解活性和产量损失。对两个耐旱（Yantar和Zlatitsa）和两个干旱敏感（Miziya和Dobrudjanka）冬小麦品种的旗叶进行了分析。胁迫强度通过相对电解质渗漏率和脯氨酸积累来评估。衰老进程其次是叶绿素和蛋白质的损失。从抽穗期到灌浆期，对小麦籽粒膜脂过氧化、H2 O2含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和非特异性过氧化物酶（GPX）同工酶活性以及蛋白水解活性进行了分析。在田间干旱条件下，敏感品种的膜完整性减弱和脂质氧化损伤更为明显。Fe-和Cu/Zn SOD同工酶的活性在对照中下降，但在干旱处理的植物中保持较高。MnSOD同工酶和CAT活性随灌浆进程而增强，尤其是在干旱胁迫下敏感品种。GPX活性在干旱条件下升高，但逐渐降低。田间干旱条件下的加速衰老与较高的蛋白水解活性有关，蛋白酶反应具有品种特异性差异，但与抗旱性或敏感性无明显相关性。田间干旱导致更高的氧化胁迫更明显的干旱敏感品种，特别是在灌浆期。
Field drought studies were performed in order to assess oxidative stress, proteolytic activity and yield loss under natural stress conditions. Flag leaves of two drought-tolerant (Yantar and Zlatitsa) and two drought-sensitive (Miziya and Dobrudjanka) winter wheat varieties were analyzed. Stress intensity was assessed by relative electrolyte leakage and proline accumulation. Senescence progression was followed by loss of chlorophyll and protein. Lipid peroxidation, H2O2 content, activities of superoxide dismutase (SOD), catalase (CAT), and non-specific peroxidase (GPX) isoforms, as well as proteolytic activities were analyzed from heading throughout grain filling. Weakening of membrane integrity and oxidative damage to lipids were more pronounced in the sensitive varieties under field drought. The activities of Fe- and Cu/Zn SOD isoforms decreased in the controls, but remained high in drought-treated plants. The activities of MnSOD isoforms and CAT were enhanced towards grain filling, especially in the sensitive varieties under drought. GPX activities were rised under drought but progressively diminished. Accelerated senescence under field drought was linked to higher proteolytic activity with variety specific differences in the protease response, but without a clear correlation to drought resistance or sensitivity. Field drought led to higher oxidative stress more pronounced for drought sensitive varieties, especially during the grain filling period.