The cadherin Fat2 is required for planar cell polarity in the Drosophila ovary
The cadherin Fat2 is required for planar cell polarity in the Drosophila ovary
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DOI：
10.1242/dev.039099
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发表时间：
2009-12-15
期刊：
影响因子：
4.6
通讯作者：
Dahmann, Christian
中科院分区：
文献类型：
--
作者：
Viktorinova, Ivana;Koenig, Tina;Dahmann, Christian
平面细胞极性是许多上皮细胞的重要特征。在果蝇的翅膀、眼睛和腹部，平面细胞极性的建立需要核心平面细胞极性基因和两种钙粘蛋白Fat和Dachsous。果蝇Fat 2是一种与Fat相关的钙粘蛋白;然而，其在平面细胞极性中的作用尚未研究。在这里，我们已经产生了突变的脂肪2，并表明，脂肪2是所需的肌动蛋白丝的平面极性取向在基底侧的卵泡细胞。肌动蛋白丝方向的缺陷与卵子发生过程中卵室不能伸长有关。使用一个功能性的fosmid为基础的fat 2-GFP转基因，我们表明，在卵泡细胞中的分布Fat 2蛋白是平面极化和Fat 2定位于基础肌动蛋白丝终止。镶嵌分析表明，Fat 2的行为在卵泡细胞的非自主性，表明Fat 2的极性信息的传输所需的。我们的研究结果表明，脂肪样钙粘蛋白在平面细胞极性的建立过程中的主要作用。
Planar cell polarity is an important characteristic of many epithelia. In the Drosophila wing, eye and abdomen, establishment of planar cell polarity requires the core planar cell polarity genes and two cadherins, Fat and Dachsous. Drosophila Fat2 is a cadherin related to Fat; however, its role during planar cell polarity has not been studied. Here, we have generated mutations in fat2 and show that Fat2 is required for the planar polarity of actin filament orientation at the basal side of ovarian follicle cells. Defects in actin filament orientation correlate with a failure of egg chambers to elongate during oogenesis. Using a functional fosmid-based fat2-GFP transgene, we show that the distribution of Fat2 protein in follicle cells is planar polarized and that Fat2 localizes where basal actin filaments terminate. Mosaic analysis demonstrates that Fat2 acts non-autonomously in follicle cells, indicating that Fat2 is required for the transmission of polarity information. Our results suggest a principal role for Fat-like cadherins during the establishment of planar cell polarity.