Imaging cytokine targeting to the tumor/bone microenvironment in vivo.
Imaging cytokine targeting to the tumor/bone microenvironment in vivo.
复制标题
针对体内肿瘤/骨微环境的细胞因子成像。
DOI：
10.1089/humc.2014.085
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Figueiredo,MarxaL
中科院分区：
文献类型：
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作者：
Ellis,Jane;Falzon,Miriam;Emrick,Todd;Figueiredo,MarxaL
图1.肿瘤或骨靶向多肽修饰C末端的Gaussia荧光素酶定位于前列腺肿瘤。(A)肿瘤靶向Gluc细胞因子模型的超声传递示意图和肌肉分泌。(B)TC2R-Luc标记的前列腺肿瘤细胞在第0天植入胫骨，第3天给出可检测到的信号。表达Gluc(PGL)的质粒在第4天通过超声波传递到肌肉(IM)，(D)第7天的体外成像显示，只有靶向的pGluc在肿瘤/骨转移中显示信号。(E)在超声照射后第7天或第18天，肿瘤/骨与正常器官的折叠GLuc信号高于对照。此外，与正常器官相比，#p<；0.04；在第18天，#p<；0.03Tu/骨，而在第7天，#p<；0.03Tu/bone信号。Ctrl指的是PGL的传递，PGL是一种表达赤子荧光素酶的质粒，含有Gluc，带有扰乱的、非特异性的肽序列。彩色图像可在线获取，网址为www..LiebertpubCOM/HUMC
FIG. 1. Gaussia luciferase modified at the C-terminus with tumor or bone targeting peptides localize to intratibial prostate tumors.(A) Sonodelivery schematic and muscle secretion of tumor-targeted Gluc cytokine model.(B) TC2R-Luc-marked prostate tumor cells implanted in tibia on day 0 give a detectable signal by day 3. Plasmids expressing Gluc (pGL) are delivered intramuscularly (IM) via sonodelivery on day 4, and mice imaged for GLuc expression at day 7 (C).(D) Ex vivo imaging on day 7 shows that only targeted pGluc show signals in tumor/bone metastases.(E) Quantification of fold GLuc signal over control in tumor/bone versus normal organs on day 7 or 18 postsonoporation. In addition,* p< 0.04 compared with normal organs;# p< 0.03 Tu/bone on day 18 compared with Tu/bone signals on day 7. Ctrl refers to delivery of pGL, a plasmid expressing Gaussia luciferase bearing Gluc with a scrambled, nonspecific peptide sequence. Color images available online at www. liebertpub. com/humc