Ion/ion reactions in the gas phase: Proton transfer reactions involving multiply-charged proteins
Ion/ion reactions in the gas phase: Proton transfer reactions involving multiply-charged proteins
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DOI：
10.1021/ja9611755
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发表时间：
1996-08-07
影响因子：
15
通讯作者：
McLuckey, SA
中科院分区：
文献类型：
--
作者：
Stephenson, JL;McLuckey, SA
来自牛泛素和马骨骼肌脱辅基肌红蛋白的电喷雾的多电荷的阳离子已经进行了与来自全氟-1，3-二甲基环己烷的辉光放电电离的阴离子的反应。结果进行了比较，从质子转移反应与强气态中性碱的数据。离子/离子反应速率被证明是线性相关的蛋白质离子上的电荷的平方，如预期的基础上一个简单的捕获碰撞模型。具有低至+1电荷的阳离子产物可以通过离子/离子反应容易地形成，而通过离子/分子反应产生这种低电荷状态的努力已被证明是不成功的。离子/离子质子转移反应似乎是将高电荷蛋白质上的电荷减少到任意低电荷状态的有效手段。除了质子转移，还观察到离子/离子复合。质子转移相对于阴离子附着到阳离子的倾向高度依赖于阴离子的身份。
Multiply-charged cations derived from electrospray of bovine ubiquitin and horse skeletal muscle apomyoglobin have been subjected to reactions with anions derived from glow discharge ionization of perfluoro-1,3-dimethylcyclohexane. The results are compared with data obtained from proton transfer reactions with strong gaseous neutral bases. Ion/ion reaction rates are shown to be linearly related to the square of the charge on the protein ion, as expected based on a simple capture collision model. Cationic products with charge as low as +1 could be readily formed via ion/ion reactions, whereas efforts to produce such low charge states via ion/molecule reactions have proved unsuccessful. Ion/ion proton transfer reactions appear to be an effective means of reducing charge on highly charged proteins to arbitrarily low charge states. In addition to proton transfer, ion/ion recombination has also been observed. The propensity for proton transfer versus anion attachment to the cation is highly dependent upon the identity of the anion.