Microneedle arrays allow lower microbial penetration than hypodermic needles in vitro.
Microneedle arrays allow lower microbial penetration than hypodermic needles in vitro.
复制标题
DOI：
10.1007/s11095-009-9967-2
复制
发表时间：
2009-11
影响因子：
3.7
通讯作者：
Woolfson, A. David
中科院分区：
文献类型：
--
作者：
Donnelly, Ryan F.;Singh, Thakur Raghu Raj;Tunney, Michael M.;Morrow, Desmond I. J.;McCarron, Paul A.;O'Mahony, Conor;Woolfson, A. David
在这项研究中，我们首次使用两种不同的体外模型确定了微生物穿过微针诱导的孔的能力。当使用 Silescol® 膜时，当膜被微针而不是 21G 皮下注射针刺穿时，穿过膜的白色念珠菌、铜绿假单胞菌和表皮葡萄球菌的数量要低一个数量级。除了白色念珠菌穿过皮下针刺膜的移动（其中对照膜上的微生物负荷的 40.2% 在 24 小时内渗透过屏障）之外，渗透微生物的数量还不到对照膜上原始微生物负荷的 5%。使用切除的猪皮和放射性标记微生物的实验表明，穿透皮肤超出角质层的微生物数量比相应实验中穿过 Silescol® 膜的微生物数量大约大一个数量级。在插入过程中，每种微生物大约有 103cfu 粘附在皮下注射针上。在每种情况下，附着在 MN 阵列上的微生物数量都高出一个数量级。我们在此表明，与皮下针穿刺相比，微针穿刺导致的微生物渗透显着减少，并且与针穿刺皮肤相比，微针穿刺皮肤中没有微生物穿过活表皮。因此，鉴于皮肤的抗菌特性，以适当的方式将微针阵列应用于皮肤可能不会在免疫功能正常的患者的正常情况下引起局部或全身感染。为了支持基于微针的输送系统的广泛临床使用，现在需要适当的动物研究来最终证明体内这一点。通过无菌或无菌制造以及用自失能材料（例如可溶解或可生物降解的聚合物）制造微针以防止不当或意外重复使用，可以提高患者的安全性。
In this study we determined, for the first time, the ability of microorganisms to traverse microneedle-induced holes using two different in vitro models. When employing Silescol® membranes, the numbers of Candida albicans, Pseudomonas aeruginosa and Staphylococcus epidermidis crossing the membranes were an order of magnitude lower when the membranes were punctured by microneedles rather than a 21G hypodermic needle. Apart from the movement of C. albicans across hypodermic needle-punctured membranes, where 40.2% of the microbial load on control membranes permeated the barrier over 24 h, the numbers of permeating microorganisms was less than 5% of the original microbial load on control membranes. Experiments employing excised porcine skin and radiolabelled microorganisms showed that the numbers of microorganisms penetrating skin beyond the stratum corneum were approximately an order of magnitude greater than the numbers crossing Silescol® membranes in the corresponding experiments. Approximately 103cfu of each microorganism adhered to hypodermic needles during insertion. The numbers of microorganisms adhering to MN arrays were an order of magnitude higher in each case. We have shown here that microneedle puncture resulted in significantly less microbial penetration than did hypodermic needle puncture and that no microorganisms crossed the viable epidermis in microneedle—punctured skin, in contrast to needle-punctured skin. Given the antimicrobial properties of skin, it is, therefore, likely that application of microneedle arrays to skin in an appropriate manner would not cause either local or systemic infection in normal circumstances in immune-competent patients. In supporting widespread clinical use of microneedle-based delivery systems, appropriate animal studies are now needed to conclusively demonstrate this in vivo. Safety in patients will be enhanced by aseptic or sterile manufacture and by fabricating microneedles from self-disabling materials (e.g. dissolving or biodegradable polymers) to prevent inappropriate or accidental reuse.
登录
查看更多内容
影响因子：
6.5
作者：
Wang, Ping M.;Cornwell, Megan;Prausnitz, Mark R.
通讯作者：
Prausnitz, Mark R.
影响因子：
5.5
作者：
Van Damme, Pierre;Oosterhuis-Kafeja, Froukje;Levin, Yotam
通讯作者：
Levin, Yotam
DOI：
10.1073/pnas.2331316100
发表时间：
2003-11-25
影响因子：
11.1
作者：
McAllister, DV;Wang, PM;Prausnitz, MR
通讯作者：
Prausnitz, MR
影响因子：
10.8
作者：
Park, JH;Allen, MG;Prausnitz, MR
通讯作者：
Prausnitz, MR
影响因子：
2.8
作者：
Haq, M. I.;Smith, E.;Birchall, J. C.
通讯作者：
Birchall, J. C.