Determination of in vitro antioxidant and radical scavenging activities of propofol
Determination of in vitro antioxidant and radical scavenging activities of propofol
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DOI：
10.1248/cpb.53.281
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发表时间：
2005-03-01
影响因子：
1.7
通讯作者：
Cesur, M
中科院分区：
文献类型：
--
作者：
Gülçin, I;Alici, HA;Cesur, M
丙泊酚（2，6-二异丙基苯酚）是一种具有体内抗氧化特性的静脉内催眠药。本研究采用1，1-二苯基-2-苦基-肼（DPPH中心点）自由基清除、金属螯合、过氧化氢清除、超氧阴离子自由基清除、还原力和总抗氧化能力等抗氧化试验，考察丙泊酚的体外抗氧化活性。在25、50和75 μ g/ml浓度下，丙泊酚对亚油酸乳剂过氧化反应的抑制率分别为97.7%、98.6%和100%。另一方面，在75 μ g/ml浓度下，标准抗氧化剂如丁基化羟基茴香醚（BHA）、丁基化羟基甲苯（BHT）和α-生育酚对亚油酸乳液的过氧化分别表现出88.7%、94.5%和70.4%的抑制。此外，在相同浓度下，丙泊酚具有有效的还原力、清除DPPH中心点自由基、清除超氧阴离子自由基、清除过氧化氢和金属螯合活性。将这些不同的抗氧化剂活性与标准抗氧化剂如BHA、BHT和α-生育酚进行比较。这些结果表明，异丙酚防止脂质过氧化和自由基链反应。与此同时，丙泊酚显示出比BHA、BHT和α-生育酚更有效的抗氧化能力。
Propofol (2,6-diisopropylphenol) is a hypnotic intravenous agent with in vivo antioxidant properties. This study was undertaken to examine the in vitro antioxidant activity of propofol using different antioxidant tests including by 1,1-diphenyl-2-picryl-hydrazil (DPPH center dot) radical scavenging, metal chelating, hydrogen peroxide scavenging, superoxide anion radical scavenging, reducing power and total antioxidant activities. At the concentrations of 25, 50, and 75,mu g/ml, propofol exhibited 97.7, 98.6 and 100% inhibition on peroxidation of linoleic acid emulsion, respectively. On the other hand, at the 75,mu g/ml concentration of standard antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and alpha-tocopherol exhibited 88.7, 94.5, and 70.4% inhibition on peroxidation of linoleic acid emulsion, respectively. In addition, at same concentrations, propofol was shown that it had effective reducing power, DPPH center dot free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging and metal chelating activities. These various antioxidant activities were compared to standard antioxidants such as BHA, BHT and alpha-tocopherol. These results indicate that propofol prevents lipid peroxidation and radicalic chain reactions. At the same time, propofol revealed more effective antioxidant capacity than BHA, BHT and alpha-tocopherol.