LYSOSOMAL VACUOLAR APPARATUS OF CARDIAC MYOCYTES IN HEART OF STARVED AND REFED RABBITS
LYSOSOMAL VACUOLAR APPARATUS OF CARDIAC MYOCYTES IN HEART OF STARVED AND REFED RABBITS
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DOI：
10.1016/0022-2828(80)90064-4
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发表时间：
1980-01-01
影响因子：
5
通讯作者：
WILDENTHAL, K
中科院分区：
文献类型：
--
作者：
DECKER, RS;DECKER, ML;WILDENTHAL, K
采用生化、免疫组织化学和细胞化学技术研究了长时间饥饿和再喂养后心脏溶酶体的变化。饥饿期溶酶体酶活性呈异质性变化：组织蛋白酶D、酸性磷酸酶和β -酶显著升高。-氨基葡萄糖酶变化最小，组织蛋白酶B显著降低。复饲1 ~ 3 d，除酸性磷酸酶外，其他各项活性均恢复正常。形态学研究表明，组织蛋白酶D和酸性磷酸酶的增加局限于内质网、高尔基复合体和次级溶酶体的元素；再饲喂后，这些酸性水解酶被纳入自噬液泡中，同时内质网内的染色丧失。在饥饿状态下，不同的心脏溶酶体蛋白酶显然不以协调的方式调节。组织蛋白酶D的变化似乎在重新进食期间比在饥饿期间本身更有可能发挥重要的代谢作用。
The cardiac lysosomal changes that accompany prolonged starvation and refeeding were studied using biochemical, immunohistochemical and cytochemical techniques. Biochemical activities of lysosomal enzymes changed in a heterogeneous manner during starvation: cathepsin D increased significantly, acid phosphatase and .beta.-glucosaminidase were altered minimally and cathepsin B decreased significantly. During 1-3 days of refeeding, all activities returned toward normal except acid phosphatase. Morphological studies revealed that the increases in cathepsin D and acid phosphatase were localized in elements of the endoplasmic reticulum, Golgi complex and secondary lysosomes; upon refeeding these acid hydrolases were incorporated within autophagic vacuoles with simultaneous loss of staining within the endoplasmic reticulum. Different cardiac lysosomal proteinases evidently are not regulated in a coordinate manner during starvation. Changes in cathepsin D appear more likely to play an important metabolic role during the period of refeeding than during the period of starvation per se.