DNA methylation status of DNAJA4 is essential for human erythropoiesis
DNA methylation status of DNAJA4 is essential for human erythropoiesis
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DNAJA4 的 DNA 甲基化状态对于人类红细胞生成至关重要
DOI：
10.2217/epi-2022-0341
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发表时间：
2022
期刊：
影响因子：
3.8
通讯作者：
Xiuyun Wu
中科院分区：
文献类型：
--
作者：
Hengchao Zhang;Fumin Xue;Huizhi Zhao;Lixiang Chen;Ting Wang;Xiuyun Wu
目的：目的研究红细胞生成早期和终末阶段的DNA甲基化模式，探讨差异甲基化基因在红细胞生成和红系疾病中的作用。材料方法：对急性髓系白血病进行了红细胞生成过程中DNA甲基化和基因表达的差异分析以及加权基因共表达网络分析。结果：我们确定了四个候选基因，具有差异甲基化的启动子区域。DNAJA 4影响终末红细胞生成过程中的增殖、凋亡和去核，并与急性髓性白血病的预后相关。DNAJA4在红白血病中特异性高表达，并与DNA甲基化相关。结论：DNAJA4在红细胞生成中起重要作用，并受DNA甲基化调控。DNAJA4表达失调与红系疾病有关。
Aims: To investigate DNA methylation patterns in early and terminal stages of erythropoiesis, and to explore the function of differentially methylated genes in erythropoiesis and erythroid disorders. Materials methods: Differential analysis of DNA methylation and gene expression during erythropoiesis, as well as weighted gene coexpression network analysis of acute myeloid leukemia was performed. Results: We identified four candidate genes that possessed differential methylation in the promoter regions. DNAJA4 affected proliferation, apoptosis and enucleation during terminal erythropoiesis and was associated with the prognosis of acute myeloid leukemia. DNAJA4 was specifically highly expressed in erythroleukemia and is associated with DNA methylation. Conclusion: DNAJA4 plays a crucial role for erythropoiesis and is regulated via DNA methylation. Dysregulation of DNAJA4 expression is associated with erythroid disorders.