Highly elastomeric photocurable silk hydrogels
Highly elastomeric photocurable silk hydrogels
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DOI：
10.1016/j.ijbiomac.2019.05.068
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发表时间：
2019-08-01
影响因子：
8.2
通讯作者：
Lu, Shenzhou
中科院分区：
文献类型：
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作者：
Kuang, Dajiang;Jiang, Fujian;Lu, Shenzhou
首次以天然丝素蛋白和生物光敏剂核黄素为原料制备了可光固化的丝素蛋白水凝胶。核黄素被紫外光激发产生三重态，三重态被转移产生以单线态氧为主要成分的活性氧自由基。活性氧自由基可以诱导丝素蛋白大分子中的氨基、苯酚等基团发生化学交联，形成光固化水凝胶。采用傅里叶变换红外光谱、X射线衍射、扫描电镜、酶标仪和质构仪等分析手段，研究了该改性丝素蛋白溶液凝胶化的不同生物物理特性。研究了凝胶的聚集体结构、表面形貌、力学性能、透光性和降解性能。研究表明，丝素蛋白/核黄素水凝胶主要具有无规卷曲或α螺旋结构。这些凝胶在80%压缩后显示出高达90%的弹性和高达97%的透光率。细胞培养实验表明，该水凝胶具有良好的细胞相容性。(C)2019由Elsevier B. V.出版
A photocurable silk fibroin hydrogel is prepared, for the first time, using natural silk protein fibroin and biophotosensitizer riboflavin. Riboflavin is excited by ultraviolet light to generate a triplet state which is transferred to produce active oxygen radicals with singlet oxygen as the main component. Active oxygen radicals can induce chemical cross-linking of amino-, phenol- and other groups in the silk fibroin macromolecules to form a photocurable hydrogel. The different biophysical characterizations of the gelation of this modified fibroin protein solution were studied by using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, microplate reader and texture analyzer. The aggregate structures, surface morphologies, mechanical properties, light transmission and degradation properties of the gel were studied. The investigations showed that the silk fibroin/riboflavin hydrogels predominantly have random coils or alpha helix structures. These gels show resilience up to 90% after 80% compression and a light transmission of up to 97%. The cell culture experiment exhibits that the hydrogel has a satisfactory cytocompatibility. (C) 2019 Published by Elsevier B.V.