Cells on microspheres: a new technique for flow cytometric analysis of adherent cells.
Cells on microspheres: a new technique for flow cytometric analysis of adherent cells.
复制标题
微球上的细胞：贴壁细胞流式细胞分析的新技术。
DOI：
10.1002/cyto.990030611
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Ault,KA
中科院分区：
文献类型：
--
作者：
Bloch,DB;Smith,BR;Ault,KA
此前，技术问题阻碍了荧光激活细胞分选技术在贴壁细胞群研究中的应用。我们开发了一种将人类单核巨噬细胞附着到 14-20 微米微球上的程序。微球上的这些贴壁细胞保留了吞噬能力，可以使用间接免疫荧光对细胞表面抗原进行染色，并且可以在长期培养中维持。它们可以在仍处于贴壁状态时在荧光激活细胞分选仪上进行检查和分选。该技术有利于贴壁细胞的流式细胞术研究，并允许分离这些细胞的亚群。
Technical problems have previously prevented the application of fluorescence activated cell sorting to the study of adherent cell populations. We have developed a procedure for attachment of human monocytemacrophages to 14–20 micrometer microspheres. These adherent cells on microspheres retained phagocytic capacity, could be stained for cell surface antigens using indirect immunofluorescence, and could be maintained in long‐term culture. They could be examined and sorted on a fluorescence activated cell sorter while still in the adherent state. This technique facilitates the flow cytometric study of adherent cells and permits the isolation of subpopulations of these cells.