Quantitative Detection of Epstein-Barr Virus in Bronchoalveolar Lavage From Transplant and Nontransplant Patients
Quantitative Detection of Epstein-Barr Virus in Bronchoalveolar Lavage From Transplant and Nontransplant Patients
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DOI：
10.1097/tp.0b013e3181890415
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发表时间：
2008-11-27
期刊：
影响因子：
6.2
通讯作者：
Cavallo, Rossana
中科院分区：
文献类型：
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作者：
Costa, Cristina;Elia, Mariateresa;Cavallo, Rossana
背景下呼吸道是EB病毒（EBV）的潜伏部位;然而，其致病作用知之甚少，特别是在移植患者中。本研究的目的是评估EB病毒在移植受者（TR）和非移植（NT）患者支气管肺泡灌洗液（BAL）中的患病率和作用。对194例患者（TR组59例107例，NT组143例165例）的272份连续BAL标本进行EB病毒、人类疱疹病毒6型（HHV-6）和人类疱疹病毒7型（HHV-7）实时定量聚合酶链反应（PCR）和人巨细胞病毒（HCMV）快速壳式培养。EBV-DNA阳性率为29.4%（65/57），其中TR组为40.7%（24/59），NT组为23.1%（33/143）（P
Background. The lower respiratory tract is a latency site of Epstein-Barr virus (EBV); however, its pathogenic role is poorly known, particularly in transplant patients. The aim of this Study was to evaluate the prevalence and role of EBV in bronchoalveolar lavages (BAL) from transplant recipients (TR) in comparison with nontransplant (NT) patients.Methods. Real-time quantitative polymerase chain reaction for EBV, human herpesvirus-6 (HHV-6), and HHV-7 and rapid shell-via] culture for human cytomegalovirus (HCMV) were performed on 272 consecutive BAL from 194 patients (107 from 59 TR and 165 from 143 NT).Results. EBV-DNA was positive in 65 specimens (23.9%) from 57 patients (29.4%): 24 of 59 (40.7%) TR and 33 of 143 (23.1%) NT (P