Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle naturally infected with Mycobacterium bovis in Ethiopia
Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle naturally infected with Mycobacterium bovis in Ethiopia
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埃塞俄比亚自然感染牛分枝杆菌的无症状牛肉芽肿的细胞和细胞因子反应
DOI：
10.1101/2020.06.12.149518
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Tulu B
中科院分区：
文献类型：
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作者：
Tulu B
在自然感染牛分枝杆菌的牛的淋巴结和组织中评价了细胞（CD 3 +T细胞和CD 68+巨噬细胞）、细胞因子（干扰素γ阳性[IFN-γ+]和肿瘤坏死因子α阳性[TNF-α+]）和效应分子（诱导型一氧化氮合酶阳性[iNOS+]）应答。详细的尸检和免疫组化检查病变进行了16头奶牛，是阳性的单皮内宫颈比较结核菌素（SICCT）试验，并确定从奶牛场周围的城市阿迪斯，埃塞俄比亚。M组的大体病变严重程度显著高于M组（P= 0.003）。牛培养阳性奶牛（n= 12）比培养阴性奶牛（n= 4）。免疫组化结果显示，在培养阳性的奶牛中，随着肉芽肿分期从I期到IV期的增加，CD 3 +T细胞的平均免疫标记分数降低（P< 0.001）。与此相反，从I期到IV期，CD 68+巨噬细胞、IFN-γ+、TNF-α+和iNOS+免疫标记分数增加（P< 0.001）。在早期阶段，培养阴性的奶牛表现出显着较高的分数的CD 68+巨噬细胞（P= 0.03）和iNOS+（P= 0.007）免疫标记分数比培养阳性的奶牛。同样，在晚期肉芽肿阶段，培养阴性奶牛的CD 3 +T细胞平均比例显著高于培养阳性奶牛（P< 0.001）。因此，本研究表明，在自然感染奶牛M。随着肉芽肿分期从Ⅰ期增加到Ⅳ期，CD 3+细胞的免疫标记分数减少，而CD 68+巨噬细胞、IFN-γ+、TNF-α+和iNOS+免疫标记分数增加。
Cell (CD3+T cell and CD68+macrophages), cytokine (interferon gamma-positive [IFN-γ+] and tumor necrosis factor alpha-positive [TNF-α+]), and effector molecule (inducible nitric oxide synthase-positive [iNOS+]) responses were evaluated in the lymph nodes and tissues of cattle naturally infected with Mycobacterium bovis. Detailed postmortem and immunohistochemical examinations of lesions were performed on 16 cows that were positive by the single intradermal cervical comparative tuberculin (SICCT) test and that were identified from dairy farms located around the city of Addis Ababa, Ethiopia. The severity of the gross lesion was significantly higher (P= 0.003) in M. bovis culture-positive cows (n= 12) than in culture-negative cows (n= 4). Immunohistochemical techniques showed that in culture-positive cows, the mean immunolabeling fraction of CD3+T cells decreased as the stage of granuloma increased from stage I to stage IV (P< 0.001). In contrast, the CD68+macrophage, IFN-γ+, TNF-α+, and iNOS+immunolabeling fractions increased from stage I to stage IV (P< 0.001). In the early stages, culture-negative cows showed a significantly higher fraction of CD68+macrophage (P= 0.03) and iNOS+(P= 0.007) immunolabeling fractions than culture-positive cows. Similarly, at advanced granuloma stages, culture-negative cows demonstrated significantly higher mean proportions of CD3+T cells (P< 0.001) than culture-positive cows. Thus, this study demonstrates that, following natural infection of cows with M. bovis, as the stage of granuloma increases from stage I to stage IV, the immunolabeling fraction of CD3+cells decreases, while the CD68+macrophage, IFN-γ+, TNF-α+, and iNOS+immunolabeling fractions increases.