Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray
Long Noncoding RNA Expression Signatures of Abdominal Aortic Aneurysm Revealed by Microarray
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微阵列揭示腹主动脉瘤的长非编码 RNA 表达特征
DOI：
10.3967/bes2016.096
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发表时间：
2016-10-01
影响因子：
3.5
通讯作者：
Chen Zhong
中科院分区：
文献类型：
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作者：
Yang Yao Guo;Li Mei Xia;Chen Zhong
目的探讨长链非编码RNA（lncRNA）在腹主动脉瘤（AAA）发病机制中的作用。方法采用基因芯片技术分析AAA组织和正常腹主动脉组织（NT）中lncRNA和mRNA的表达特征，并通过实时定量逆转录PCR（qRT-PCR）进行验证。使用计算分析鉴定lncRNAs-mRNAs靶向关系。结果在AAA和NT组织中，共检测到3,688条lncRNA和3,007条mRNA的差异表达。此外，通过生物信息学分析，识别出1,284个差异表达的长基因间非编码RNA和206个与蛋白质编码基因相邻的差异表达的增强子样lncRNA。采用qRT-PCR进一步验证AAA和正常组织样本之间的一些差异表达的lncRNA和mRNA。通过对差异表达的lncRNA和mRNA进行相关性分析，构建了编码基因和非编码基因的共表达网络。此外，通过使用计算分析蛋白质编码潜力，将位于ALOX 5上游的lnc-ARG分类为非编码转录物。此外，lnc-ARG还能降低HeLa细胞ALOX 5的mRNA水平和活性氧的产生。结论新的lncRNA候选物与AAA的发病机制有关。
Objective This study is aimed at observing the role of long noncoding RNAs (lncRNAs) in the pathogenesis of abdominal aortic aneurysm (AAA).Methods LncRNA and mRNA expression signatures of AAA tissues and normal abdominal aortic tissues (NT) were analyzed by microarray and further verified by Real-time quantitative reverse-transcription PCR (qRT-PCR). The lncRNAs-mRNAs targeting relationships were identified using computational analysis. The effect of lnc-ARG on 5-lipoxygenase (ALOX5) expression was tested in HeLa cells.Results Differential expressions of 3,688 lncRNAs and 3,007 mRNAs were identified between AAA and NT tissues. Moreover, 1,284 differentially expressed long intergenic noncoding RNAs and 206 differentially expressed enhancer-like lncRNAs adjacent to protein-coding genes were discerned by bioinformatics analysis. Some differentially expressed lncRNAs and mRNAs between AAA and normal tissue samples were further verified using qRT-PCR. A co-expression network of coding and noncoding genes was constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. In addition, the lnc-ARG located within the upstream of ALOX5 was sorted as a noncoding transcript by analyzing the protein-coding potential using computational analysis. Furthermore, we found that lnc-ARG can decrease the mRNA level of ALOX5 and reactive oxygen species production in HeLa cells.Conclusion This study revealed new lncRNA candidates are related to the pathogenesis of AAA.