ANTIBODY FORMATION IN VITRO
ANTIBODY FORMATION IN VITRO
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DOI：
10.1084/jem.114.6.837
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发表时间：
1961-01-01
影响因子：
15.3
通讯作者：
FISHMAN, M
中科院分区：
文献类型：
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作者：
FISHMAN, M
对T2噬菌体的中和活性出现在正常大鼠的淋巴结细胞培养物中，以响应其在体外用来自与T2噬菌体一起孵育的均质化大鼠巨噬细胞的无细胞滤液刺激。该活性特异性针对T2噬菌体。它存在于具有血清球蛋白的盐析特性的培养液的一部分中。鼠血清丙种球蛋白特异性抗体可抑制噬菌体中和。如果（1）单独加入T2噬菌体，（2）如果过度缩短巨噬细胞和T2噬菌体的孵育期，（3）如果将无细胞滤液在80-100 ℃下加热15分钟，（4）如果向巨噬细胞中加入超过最佳量的T2噬菌体。阻止抗体形成的其他因素是淋巴结细胞的热灭活或向培养基中添加链霉素或核糖核酸酶。最后，发现巨噬细胞和淋巴结细胞必须从同一物种的动物中获得。用鸡胚代替组织培养基，可以证实体外产生抗T2噬菌体抗体的所有基本发现。结果进行了讨论，在一个可能的机制，抗体的生产中，从巨噬细胞和抗原的相互作用产生的RNA酶敏感的物质是能够刺激抗体的合成在淋巴细胞。
Neutralizing activity against T2 bacteriophage appeared in cultures of lymph node cells from normal rats in response to their in vitro stimulation with a cell-free filtrate derived from homogenized rat macrophages which had been incubated with T2 bacteriophage. This activity was specifically directed against T2 bacteriophage. It resided in a fraction of the culture fluid which had the salting-out properties of serum globulin. Phage neutralization was inhibited by antibody specific for rat serum gamma globulin. Antibody production against T2 bacteriophage in cultures of lymph node cells from normal animals failed to occur if (1) T2 bacteriophage alone was added, (2) if the incubation period of macrophages and T2 phage was unduly shortened, (3) if the cell-free filtrate was heated at 80-100[degree]C for 15 minutes, (4) if more than an optimal amount of T2 bacteriophage was added to the macrophages. Additional factors which prevented the formation of antibody were the heat inactivation of the lymph node cells or the addition to the culture medium of either streptomycin or ribonuclease. Finally, it was found that macrophages and lymph node cells had to be obtained from animals of one and the same species. All essential findings on the production of antibody to T2 bacteriophage in vitro could be confirmed by substitution of the chick embryo for the tissue culture medium. The results are discussed in terms of a possible mechanism of antibody production in which an RNAse-sensitive substance resulting from the interaction of macrophages and antigen is capable of stimulating antibody synthesis in lymphocytic cells.