Response of soil bacterial community to repeated applications of carbendazim.
Response of soil bacterial community to repeated applications of carbendazim.
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DOI：
10.1016/j.ecoenv.2011.08.014
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发表时间：
2012
影响因子：
6.8
通讯作者：
Xiuguo Wang;M. Song;Yi-qing Wang;Chunming Gao;Qun Zhang;X. Chu;H. Fang;Yunlong Yu
中科院分区：
文献类型：
--
作者：
Xiuguo Wang;M. Song;Yi-qing Wang;Chunming Gao;Qun Zhang;X. Chu;H. Fang;Yunlong Yu
在田间条件下研究了多菌灵重复施用对土壤可培养微生物功能多样性和细菌群落组成的影响。在0.94、1.88和4.70kg有效成分（a.i.）水平下，首次施用多菌灵后，土壤可培养微生物群落功能多样性指数（Shannon指数，H′）显著降低（P<0.05）。ha-1，然后在随后的应用中恢复到对照中的水平。温度梯度凝胶电泳（TGGE）分析表明，第2次施用多菌灵后，土壤细菌群落组成与对照相比有显著差异（P<0.01），第3次和第4次施用多菌灵后，土壤细菌群落组成仍保持不变。结果表明，多菌灵的重复施用对土壤可培养微生物群落的功能多样性具有短暂的有害影响，并导致细菌群落组成的改变，主要是由于γ-变形杆菌中的一个物种。
The effect of repeated carbendazim applications on functional diversity of culturable microorganisms and bacterial community composition was studied under field conditions. The functional diversity of soil culturable microbial community (Shannon index, H′) reduced significantly (P<0.05) after the first introduction of carbendazim at levels of 0.94, 1.88 and 4.70kg active ingredient (a.i.)ha−1and then recovered to that in the control with subsequent applications. An evident (P<0.01) difference in the bacterial community composition was observed after the second carbendazim application by Temperature Gradient Gel Electrophoresis (TGGE) analysis of 16S rRNA genes amplified from treated and control soils, which remained after the third and fourth treatments. Our results indicated that repeated carbendazim applications have a transient harmful effect on functional diversity of soil culturable microbial community and result in an alteration in bacterial community composition largely due to one species within the γ-proteobacterium.