A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting
A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting
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DOI：
10.1039/b408422e
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Wang, YM
中科院分区：
文献类型：
--
作者：
Yao, B;Luo, GA;Wang, YM
建立了一种基于重力和电力驱动的流式细胞仪和荧光激活细胞分选的新方法。在实验中，细胞在其自身重力下在直立微芯片中自发流动，通过检测区域，然后以0.55 mm s（-1）的平均速度逐个进入分选电场，并通过关闭激活程序进行荧光激活细胞分选（FACS）。为了研究微通道内单细胞在重力和电场作用下的动力学和运动学特性，建立了基于牛顿运动定律的物理和数值模型，并对其进行了优化。羟丙基甲基纤维素（HPMC）用于减少细胞组装，沉降和吸附到微通道。应用该系统对紫外线照射HeLa细胞10、20和40 min后的细胞坏死和凋亡效应进行了研究，结果表明紫外线照射引起的细胞膜损伤是导致HeLa细胞凋亡和坏死的重要因素。
A novel method based on gravity and electric force driving of cells was developed for flow cytometry and fluorescence activated cell sorting in a microfluidic chip system. In the experiments cells flowed spontaneously under their own gravity in a upright microchip, passed through the detection region and then entered into the sorting electric field one by one at an average velocity of 0.55 mm s(-1) and were fluorescence activated cell sorted (FACS) by a switch-off activation program. In order to study the dynamical and kinematic characteristics of single cells in gravity and electric field of microchannels a physical and numerical module based on Newton's Law of motion was established and optimized. Hydroxylpropylmethyl cellulose (HPMC) was used to minimize cell assembling, sedimentation and adsorption to microchannels. This system was applied to estimate the necrotic and apoptotic effects of ultraviolet (UV) light on HeLa cells by exposing them to UV radiation for 10, 20 or 40 min and the results showed that UV radiation induced membrane damage contributed to the apoptosis and necrosis of HeLa cells.