A novel role for zebrafish zic2a during forebrain development.
A novel role for zebrafish zic2a during forebrain development.
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DOI：
10.1016/j.ydbio.2008.02.029
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发表时间：
2008-05
影响因子：
2.7
通讯作者：
Nicholas A. Sanek;Y. Grinblat
中科院分区：
文献类型：
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作者：
Nicholas A. Sanek;Y. Grinblat
转录因子表达模式在胚胎发生早期为脊椎动物前脑建立了蓝图。在未来间脑中，已经确定了几个具有模式表达的基因，但它们的具体功能和它们之间的相互作用尚未得到很好的了解。我们已经发现了zic2a基因在斑马鱼前间脑形成过程中的关键作用。我们发现zic2a是丘脑前标记arx和dlx2a转录所必需的。这一功能在丘脑前发育的早期阶段是必需的。Zic基因在进化上与glis相关，glis是介导刺猬信号传导的转录因子。有趣的是，hedgehog信号通路也促进了丘脑前庭的发育。我们询问zic2a是否在斑马鱼前脑发育的背景下与刺猬信号相互作用。我们的数据显示，刺猬信号和zic2a在不同的时间起作用，因此在前脑发育过程中以平行的途径起作用。综上所述，我们的研究结果确定Zic2a是一种新的丘脑前发育调节剂，并表明它独立于刺猬信号传导发挥作用。
Patterns of transcription factor expression establish a blueprint for the vertebrate forebrain early in embryogenesis. In the future diencephalon, several genes with patterned expression have been identified, yet their specific functions and interactions between them are not well understood. We have uncovered a crucial role for one such gene, zic2a, during formation of the anterior diencephalon in zebrafish. We show that zic2a is required for transcription of the prethalamic markers arx and dlx2a. This function is required during early steps of prethalamic development, soon after its specification. zic genes are evolutionarily related to glis, transcription factors that mediate hedgehog signaling. Intriguingly, the hedgehog signaling pathway also acts to promote development of the prethalamus. We asked if zic2a interacts with hedgehog signaling in the context of forebrain development in zebrafish. Our data show that hedgehog signaling and zic2a function at different times, and therefore act in parallel pathways during forebrain development. Taken together, our results identify Zic2a as a novel regulator of prethalamic development, and show that it functions independently of hedgehog signaling.