BONE CELLS - BIOCHEMICAL + BIOLOGICAL STUDIES AFTER ENZYMATIC ISOLATION
BONE CELLS - BIOCHEMICAL + BIOLOGICAL STUDIES AFTER ENZYMATIC ISOLATION
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DOI：
10.1126/science.146.3650.1476
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发表时间：
1964-01-01
期刊：
影响因子：
56.9
通讯作者：
FEDAK, SA
中科院分区：
文献类型：
--
作者：
PECK, WA;BIRGE, SJ;FEDAK, SA
在缓冲的粗胶原酶中短期孵育大鼠颅骨，可以分离出形态完整的细胞，这些细胞吸收活性染料，含有碱性磷酸酶，并在组织培养中增殖。新鲜收获的细胞在有氧葡萄糖代谢方面与完整的骨段相似。
Short-term incubation of rat calvaria in buffered crude collagenase permitted the isolation of morphologically intact cells that absorb vital dyes, contain alkaline phosphatase and multiply in tissue culture. Freshly harvested cells were similar to whole bone segments in aerobic glucose metabolism.