Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation
Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation
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DOI：
10.1016/j.biomaterials.2008.11.036
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发表时间：
2009-03-01
期刊：
影响因子：
14
通讯作者：
Akaike, Toshihiro
中科院分区：
文献类型：
--
作者：
Yamaki, Kazumasa;Harada, Ichiro;Akaike, Toshihiro
利用固定化纤维连接蛋白（Fn）的温敏性水凝胶开发了一种新的细胞等双轴拉伸培养基质。在凝胶基质上培养的细胞随着凝胶的溶胀而等双轴拉伸，这伴随着温度的轻微变化。在凝胶溶胀过程中，由于凝胶的高透明度，通过光学显微镜可以清楚地观察到细胞形状的变化。ERK在机械刺激下被高度瞬时激活，而粘着斑激酶（FAK）则没有，表明机械信号在细胞中被转化为生化信号。我们发现，细胞形成丝状伪足样的结构，在响应机械线索，这表明，机械力促进肌动蛋白聚合在周边地区。在细胞质中，含有桩蛋白的纤维结构形成沿着肌动蛋白纤维。这些结果表明，我们的方法可以进行细胞内信号转导的分析和观察细胞的形状在高放大率。(c)2008爱思唯尔有限公司保留所有权利。
A new culture substrate was developed for cells to be equibiaxially stretched using fibronectin (Fn)-immobilized temperature-responsive hydrogel. The cells cultured on the gel substrate were equibiaxially stretched with swelling of the gel, which was accompanied by slight changes of temperature. During gel swelling, changes of cell shape were clearly observed by optical microscopy because of high transparency of the gel. ERK was highly and transiently activated by mechanical stimulation whereas focal adhesion kinase (FAK) was not, indicating that mechanical signals were transduced into biochemical signals in Cells. We found that cells formed filopodia-like structures in response to mechanical cues, suggesting that mechanical forces facilitated actin polymerization at the peripheral region. In the cytoplasm, paxillin-containing fibrous structures were formed along actin fibers. These results indicate that we can perform both analysis of intracellular signal transduction and observation of cell shapes at high magnification in our method. (c) 2008 Elsevier Ltd. All rights reserved.