Expression of macrophage migration-inhibitory factor in duodenal ulcer and its relation to Helicobacter pylori infection.
Expression of macrophage migration-inhibitory factor in duodenal ulcer and its relation to Helicobacter pylori infection.
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巨噬细胞迁移抑制因子在十二指肠溃疡中的表达及其与幽门螺杆菌感染的关系
DOI：
10.4238/2015.october.29.6
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Wang Gh
中科院分区：
文献类型：
--
作者：
Yu Xh;Qiang Zhang;Yang Xp;W. Yang;F. Dai;Z. Qian;Wang Zl;Wu Cf;Zhao Hz;Wang Gh
本研究旨在探讨巨噬细胞移动抑制因子（MIF）在十二指肠溃疡上皮细胞中的表达及其与幽门螺杆菌（Hp）感染的关系，探讨MIF表达与Hp感染在十二指肠溃疡发病中的作用。采用免疫组化和原位杂交技术检测了十二指肠溃疡伴Hp感染和不伴Hp感染患者（每组40例，实验组）和正常十二指肠球部粘膜组织（每组40例，对照组）中MIF蛋白和mRNA的表达。Hp阴性者常规治疗，Hp阳性者给予根除治疗。比较治疗前及溃疡愈合后Hp和MIF的表达水平。治疗前Hp感染者MIF蛋白和mRNA的阳性率分别为67.5%和65%，治疗后Hp清除者MIF蛋白和mRNA的阳性率分别为18.9%和21.6%。治疗前后与对照组比较差异有统计学意义（P < 0.05）。在非Hp感染者中，治疗前MIF蛋白和mRNA的阳性表达率分别为45%和47.5%，治疗后分别为32.5%和30%，差异无显著性（P > 0.05）。提示MIF与十二指肠溃疡的发生有关，Hp的存在与十二指肠粘膜MIF的表达及十二指肠溃疡的发生密切相关。
The aim of this study was to examine the expression of macrophage migration-inhibitory factor (MIF) in duodenal ulcer epithelial cells and its relation to Helicobacter pylori (Hp) infection, and to discuss the pathogenic roles of MIF expression and Hp infection in duodenal ulcer. MIF protein and mRNA expression was examined in samples from patients with duodenal ulcer with and without Hp infection (N = 40 each, experimental group), and in normal duodenal bulb mucosal tissue (N = 40, control group) using immunohistochemistry and in situ hybridization. Patients without Hp infection received routine treatment, and treatment was provided to the patients positive for Hp to eradicate Hp infection. Hp and MIF expression levels before treatment and after the ulcer had been cured were compared. The positive rates of MIF protein and mRNA in patients with Hp infection before treatment were 67.5 and 65%, respectively, and were 18.9 and 21.6% in the 37 patients from whom Hp was eliminated. These were statistically different both before and after treatment compared with controls (P < 0.05). In the patients without Hp infection, the positive rates of MIF protein and mRNA expression before (45 and 47.5%, respectively) and after (32.5 and 30%) treatment were not significantly different (P > 0.05). The results of this study suggested that MIF is related to the development of duodenal ulcer, and that the presence of Hp is closely related with the expression of MIF in the duodenal mucosa and the development of duodenal ulcer.