Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages
Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages
复制标题
DOI：
10.1016/j.febslet.2009.11.062
复制
发表时间：
2010-01-04
期刊：
影响因子：
3.5
通讯作者：
Kawasaki, Kiyoshi
中科院分区：
文献类型：
--
作者：
Kawano, Miyuki;Manabe, Takayuki;Kawasaki, Kiyoshi
脂质A的修饰是细菌适应宿主的必要条件。鼠伤寒沙门氏菌LpxR可能通过3'- o -去酰化解毒脂质A；然而，去酰化在其适应中的作用尚不清楚。在固定相中观察到lpxr依赖性3'- o -去酰化。当固定相细菌感染巨噬细胞时，lpxR-null菌株的细胞内生长低于野生型菌株。诱导型一氧化氮合酶在lpxR-null菌株感染细胞中的表达量高于野生型菌株。这些结果表明，脂质a3 '- o -去酰化有利于细胞内生长。(C) 2009年欧洲生化学会联合会。Elsevier B.V.版权所有。
Modification of lipid A is essential for bacterial adaptation to its host. Salmonella Typhimurium LpxR potentially detoxifies lipid A by 3'-O-deacylation; however, the involvement of deacylation in its adaptation remains unclear. LpxR-dependent 3'-O-deacylation was observed in the stationary phase. When macrophages were infected with stationary phase bacteria, the intracellular growth of the lpxR-null strain was lower than that of the wild-type strain. Furthermore, the expression level of inducible nitric oxide synthase was higher in the cells infected with the lpxR-null strain than in the cells infected with the wild-type strain. These results indicate that lipid A 3'-O-deacylation is beneficial for intracellular growth. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.