Adult human chondrocytes cultured in alginate form a matrix similar to native human articular cartilage
Adult human chondrocytes cultured in alginate form a matrix similar to native human articular cartilage
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DOI：
10.1152/ajpcell.1996.271.3.c742
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发表时间：
1996-09-01
影响因子：
5.5
通讯作者：
Thonar, EJMA
中科院分区：
文献类型：
--
作者：
Hauselmann, HJ;Masuda, K;Thonar, EJMA
成人软骨细胞在海藻酸珠中形成的基质由两部分组成：一层薄薄的细胞相关基质，相当于关节软骨的细胞周和区域基质；另一层更丰富的进一步移除的基质，相当于组织中的区域间基质。在培养的第30天，细胞的相对体积和绝对体积以及珠中两个基质室的每一个所占的体积几乎与天然关节软骨相同。此外，细胞相关基质中聚集蛋白的浓度与成人关节软骨相似，比进一步去除的基质室高出40倍。荧光激活的细胞分选显示，细胞相关基质部分是通过透明质酸和cd44样受体之间的相互作用建立在细胞膜上的。在培养的第9天，在[S-35]硫酸盐存在的情况下，软骨细胞在4小时脉冲中合成的聚集蛋白分子中约有25%保留在细胞相关基质中，在那里它们以半衰期(t(1/2)) = 29天翻转。大多数[S-35]聚集蛋白分子到达进一步移除的基质室，在那里它们的翻转速度要慢得多（t(1/2) > 100天）。这些结果进一步支持了一个论点，即居住在成人关节软骨基质的细胞周围和区域的聚集蛋白分子比那些居住在远离细胞的区域的聚集蛋白分子更容易被软骨细胞合成的蛋白水解酶降解。
The matrix formed by adult human chondrocytes in alginate beads is composed of two compartments: a thin rim of cell-associated matrix that corresponds to the pericellular and territorial matrix of articular cartilage and a more abundant further-removed matrix, the equivalent of the interterritorial matrix in the tissue. On day 30 of culture, the relative and absolute volumes occupied by the cells and each of the two matrix compartments in the beads were nearly identical to those in native articular cartilage. Furthermore, the concentration of aggrecan in the cell-associated matrix was similar to that in adult human articular cartilage and was similar to 40-fold higher than in the further removed matrix compartment. Fluorescence-activated cell sorting revealed that the cell-associated matrix was built on the cell membrane in part via interactions between hyaluronic acid and CD44-like receptors. Approximately 25% of the aggrecan molecules synthesized by the chondrocytes during a 4-h pulse in the presence of [S-35]sulfate on day 9 of culture were retained in the cell-associated matrix where they turned over with a half-life (t(1/2)) = 29 days. Most [S-35]aggrecan molecules reached the further removed matrix compartment where they turned over much more slowly (t(1/2) > 100 days). These results add support to the contention that aggrecan molecules residing in the pericellular and territorial areas of the adult human articular cartilage matrix are more susceptible to degradation by proteolytic enzymes synthesized by the chondrocytes than those that inhabit the interterritorial areas further removed from the cells.