Stem Leydig Cell Differentiation: Gene Expression During Development of the Adult Rat Population of Leydig Cells
Stem Leydig Cell Differentiation: Gene Expression During Development of the Adult Rat Population of Leydig Cells
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间质干细胞分化：成年大鼠间质细胞群发育过程中的基因表达
DOI：
10.1095/biolreprod.111.091850
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发表时间：
2011-12-01
影响因子：
3.6
通讯作者：
Jelinsky, Scott A.
中科院分区：
文献类型：
--
作者：
Stanley, Erin L.;Johnston, Daniel S.;Jelinsky, Scott A.
睾丸间质细胞是成年男性体内产生睾丸激素的细胞。成体间质细胞（adult Leydig cells，ALC）是由间质干细胞（stem Leydig cells，SLC）经至少两个中间细胞（progenitor Leydig cells，PLC）和未成熟间质细胞（immature Leydig cells，ILC）发育而来。微阵列基因表达被用来确定发生的转录变化，与分化的SLC到PLC，因此，与SLC进入Leydig细胞谱系;全面检查分化通过发展的ALC;并在SLC中的基因表达的模式，在一个完善的干细胞，骨髓干细胞（BSC）。我们发现，SLCs的基因表达模式更类似于BSCs的表达，BSCs是一种在男性生殖道外建立的干细胞，而不是Leydig细胞发育谱系中的任何细胞。这些结果表明，SLC具有许多其他干细胞的分子特征。通路分析表明，间质细胞从SLC到PLC的发展与粘附相关基因的表达减少和类固醇生成相关基因的表达增加有关。PLC和ILC之间以及ILC和ALC之间的基因表达变化相对最小，表明这些细胞高度相似。相比之下，SLC和ALC之间的基因表达变化相当不同。
Leydig cells are the testosterone-producing cells in the adult male. Adult Leydig cells (ALCs) develop from stem Leydig cells (SLCs) through at least two intermediate cells, progenitor Leydig cells (PLCs) and immature Leydig cells (ILCs). Microarray gene expression was used to identify the transcriptional changes that occur with the differentiation of SLCs to PLCs and, thus, with the entry of SLCs into the Leydig cell lineage; to comprehensively examine differentiation through the development of ALCs; and to relate the pattern of gene expression in SLCs to that in a well-established stem cell, bone marrow stem cells (BSCs). We show that the pattern of gene expression by SLCs was more similar to the expression by BSCs, an established stem cell outside the male reproductive tract, than to any of the cells in the Leydig cell developmental lineage. These results indicated that the SLCs have many of the molecular characteristics of other stem cells. Pathway analysis indicated that development of Leydig cells from SLCs to PLCs was associated with decreased expression of genes related to adhesion and increased expression of genes related to steroidogenesis. Gene expression changes between PLCs and ILCs and between ILCs and ALCs were relatively minimal, suggesting that these cells are highly similar. In contrast, gene expression changes between SLCs and ALCs were quite distinct.