A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis
A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis
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一种 TFPI-1 肽，可诱导细菌核酸降解，并抑制半滑舌鳎 (Cynoglossus semilaevis) 的细菌和病毒感染
DOI：
10.1016/j.fsi.2016.11.029
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发表时间：
2017-01-01
影响因子：
4.7
通讯作者：
Zhang, Min
中科院分区：
文献类型：
--
作者：
He, Shu-wen;Zhang, Jian;Zhang, Min
组织因子途径抑制物1（TFPI-1）是一种丝氨酸蛋白酶抑制剂，可抑制组织因子（TF）介导的凝血。TFPI-1的C-末端区域可以被切割掉，并被证明对广谱微生物具有抗菌作用。在先前的研究中，从半滑舌鳎（Cynoglossus semilaevis）TFPI-1中合成了C-末端肽TC 24（具有24个氨基酸），并发现其对藤黄微球菌具有抗菌作用。本研究进一步考察了TC 24的抗菌谱和作用方式，并对其体内功能进行了分析。结果表明，TC 24对金黄色葡萄球菌和创伤弧菌也具有杀菌活性。在与靶细菌细胞的相互作用过程中，TC 24破坏细胞膜的完整性，渗透到细胞质中，并诱导基因组DNA和总RNA的降解。体内研究表明，在细菌和病毒感染之前给予具有TC 24的舌鳎显著减少组织中的病原体传播和复制。这些结果表明，TC 24是一种新型的抗细菌和病毒的抗菌肽，TC 24对细菌RNA的作用为鱼类抗菌肽的作用机制提供了新的认识。此外，TC 24可能在水产养殖中对抗病原感染方面发挥重要作用。(C)2016爱思唯尔有限公司版权所有
Tissue factor pathway inhibitor 1 (TFPI-1) is a serine protease inhibitor that inhibits tissue factor (TF)-mediated coagulation. The C-terminal region of TFPI-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. In a previous study, a C-terminal peptide, TC24 (with 24 amino acids), derived from tongue sole (Cynoglossus semilaevis) TFPI-1, was synthesized and found antibacterial against Micrococcus luteus. In the present study, the antibacterial spectrum and the action mode of TC24 was further examined, and its in vivo function was analyzed. Our results showed that TC24 also possesses bactericidal activity against Staphylococcus aureus and Vibrio vulnificus. During its interaction with the target bacterial cells, TC24 destroyed cell membrane integrity, penetrated into the cytoplasm, and induced degradation of genomic DNA and total RNA. In vivo study showed that administration of tongue sole with TC24 before bacterial and viral infection significantly reduced pathogen dissemination and replication in tissues. These results indicated that TC24 is a novel antimicrobial peptide against bacterial and viral pathogens, and that the observed effect of TC24 on bacterial RNA adds new insights to the action mechanism of fish antimicrobial peptides. Moreover, TC24 may play an important role in fighting pathogenic infection in aquaculture. (C) 2016 Elsevier Ltd. All rights reserved.