Functional analysis of kisspeptin peptides in adult immature chub mackerel (Scomber japonicus) using an intracerebroventricular administration method
Functional analysis of kisspeptin peptides in adult immature chub mackerel (Scomber japonicus) using an intracerebroventricular administration method
复制标题
DOI：
10.1016/j.neulet.2013.12.072
复制
发表时间：
2014-02
影响因子：
2.5
通讯作者：
H. Ohga;S. Selvaraj;Hayato Adachi;Yui Imanaga;Mitsuo Nyuji;A. Yamaguchi;M. Matsuyama
中科院分区：
文献类型：
--
作者：
H. Ohga;S. Selvaraj;Hayato Adachi;Yui Imanaga;Mitsuo Nyuji;A. Yamaguchi;M. Matsuyama
在脊椎动物（包括硬骨鱼）中，控制促性腺激素分泌的关键层次因素是下丘脑促性腺激素释放激素（GnRH）十肽，它调节垂体促卵泡激素（FSH）和黄体生成素（LH）的释放。最近，Kiss1 基因编码的 Kisspeptins 已被证明可作为哺乳动物 GnRH 神经元的上游内源调节因子。鲭鱼（Scomber japonicus）大脑表达两个kiss基因（kiss1和kiss2），它们在季节性性腺周期中表现出性别二态性表达谱。在本研究中，我们评估了 Kisspeptin 肽在成年鲭鱼（2 岁以上）未成熟阶段诱导 ingnrh1（该物种中的促性腺激素释放激素形式）、fshβ 和 lhβ 转录变化的生物效力。将 100 ng 剂量的合成 Kiss1 十五肽 (Kiss1-15) 或 Kiss2 十二肽 (Kiss2-12) 注射到脑室内 (ICV) 区域，并在注射后 6 小时和 12 小时对大脑进行取样。在雌鱼中，注射后 12 小时，在两种 Kisspeptin 肽存在的情况下，gnrh1 水平下降。雄性鱼中没有观察到显着的变化。相比之下，与注射生理盐水的对照相比，ICV 注射 Kiss2-12（但不是 Kiss1-15）在注射后 12 小时显着增加了 fshβ 和 lhβ mRNA。这些结果表明合成的 Kiss2-12 可以诱导 nrh1 和 gths 的转录变化。
In vertebrates (including teleosts), the pivotal hierarchical factor in the control of gonadotropin secretion is the hypothalamic gonadotropin-releasing hormone (GnRH) decapeptide, which regulates the release of pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Recently, kisspeptins encoded by theKiss1gene have been shown to act as upstream endogenous regulators of GnRH neurons in mammals. The chub mackerel (Scomber japonicus) brain expresses twokissgenes (kiss1andkiss2) that show sexually dimorphic expression profiles during the seasonal gonadal cycle. In the present study, we evaluated the biological potency of kisspeptin peptides to induce transcriptional changes ingnrh1(hypophysiotropic GnRH form in this species),fshβandlhβduring the immature stage of adult chub mackerel (2+ years old). Synthetic Kiss1 pentadecapeptide (Kiss1-15) or Kiss2 dodecapeptide (Kiss2-12) at a dose of 100 ng were administered into the intracerebroventricular (ICV) region, and brains were sampled at 6 and 12 h post-injection. In female fish,gnrh1levels decreased in the presence of both kisspeptin peptides at 12 h post-injection. No significant variation was observed in male fish. In contrast, ICV administration of Kiss2-12 (but not Kiss1-15) significantly increasedfshβandlhβmRNAs at 12 h post-injection compared to a saline injected control in both sexes. These results suggested that synthetic Kiss2-12 could induce transcriptional changes ingnrh1andgths.