Heterodisaccharide 4-O-(N-acetyl-β-D-glucosaminyl)-D-glucosamine is an effective chemotactic attractant for Vibrio bacteria that produce chitin oligosaccharide deacetylase
Heterodisaccharide 4-O-(N-acetyl-β-D-glucosaminyl)-D-glucosamine is an effective chemotactic attractant for Vibrio bacteria that produce chitin oligosaccharide deacetylase
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DOI：
10.1111/j.1472-765x.2011.03083.x
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发表时间：
2011-08-01
影响因子：
2.4
通讯作者：
Nishio, T.
中科院分区：
文献类型：
--
作者：
Hirano, T.;Aoki, M.;Nishio, T.
目的：研究谷氨酰胺脱乙酰酶(4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine，GlcNAc-GlcN)对产碳水化合物酯酶家族4甲壳素寡糖脱乙酰酶弧菌趋化作用的诱导作用。结果表明，GlcNAc-GlcN对CE家族4种产COD弧菌、副溶血弧菌和溶藻弧菌具有有效的趋化作用。相比之下，在缺乏这种酶编码基因的内弧菌或弗氏弧菌中没有观察到这种现象。结论：GlcNAc-GlcN是CE家族4产COD弧菌、副溶血弧菌和溶藻弧菌的特异性趋化剂。本研究的意义和影响：首次阐明了GlcNAc-GlcN在具有产生CE家族4 COD能力的弧菌趋化中作为信号分子的作用。
Aims: To investigate the attractant effect of 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine (GlcNAc-GlcN) in the chemotaxis of Vibrio bacteria that produce carbohydrate esterase (CE) family 4 chitin oligosaccharide deacetylase (COD), an enzyme that catalyzes the production of GlcNAc-GlcN from N,N'-diacetylchitobiose (GlcNAc)(2).Methods and Results: The chemotactic effect of disaccharides from chitin on several strains of Vibrio bacteria was investigated using an agar gel lane-migration method. The results demonstrated that GlcNAc-GlcN functions as an effective chemoattractant in the CE family 4 COD-producing vibrios, Vibrio parahaemolyticus and Vibrio alginolyticus. In contrast, this phenomenon was not observed in Vibrio nereis or Vibrio furnissii, which lack genes encoding this enzyme. From transmission electron microscope observation of V. parahaemolyticus cells following the chemotaxis assay, GlcNAc-GlcN appears to stimulate polar flagellum rotation.Conclusions: GlcNAc-GlcN is a specific chemoattractant for the CE family 4 COD-producing vibrios, V. parahaemolyticus and V. alginolyticus.Significance and Impact of the Study: It was clarified for the first time that GlcNAc-GlcN functions as a signalling molecule in the chemotaxis of Vibrio bacteria that have an ability to produce CE family 4 COD, which generate GlcNAc-GlcN from (GlcNAc)(2).