An Ex Vivo Aorta Culture Model to Study Vascular Cellular Senescence.
An Ex Vivo Aorta Culture Model to Study Vascular Cellular Senescence.
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DOI：
10.1002/adbi.202300140
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发表时间：
2023-12
期刊：
影响因子：
3.7
通讯作者：
Yijie Yu;Shihui Bian;Yu Jiang;Bo Li;Xinggang Cui;Shu Ding;Zhiyin Dai;Rui Chen;Wei Zhong;W. Yuan
中科院分区：
文献类型：
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作者：
Yijie Yu;Shihui Bian;Yu Jiang;Bo Li;Xinggang Cui;Shu Ding;Zhiyin Dai;Rui Chen;Wei Zhong;W. Yuan
关于血管老化的动物研究存在一些局限性。最重要的原因之一是缺乏快速有效的血管组织老化模型。本研究将离体主动脉培养和Matrigel皮下植入相结合，建立了一种研究血管细胞衰老的新模型。使用8周龄C57 BL/6 J小鼠获得睾丸。博来霉素用于体外诱导睾丸衰老。然后，用Matrigel将睾丸移植到受体小鼠。使用蛋白质印迹法、定量聚合酶链反应和衰老相关β-半乳糖苷酶活性评价衰老。使用Luminex液体悬浮芯片检测炎性细胞因子。通过转录组测序分析RNA水平。结果显示，博来霉素组中的血管比对照组中的血管表现出显著的衰老，剥离血管外膜可以增强血管的衰老。在离体模型中，细胞因子如白细胞介素（IL-2、IL-1β和IL-6）的水平显著增加。此外，转录组测序显示56个显着差异表达的基因（DEG）在离体模型血管与自然老化的血管相比。总之，本研究为血管细胞衰老引入了一种具有成本效益和节省时间的血管衰老模型。
Animal studies on vascular aging pose a few limitations. One of the most important reasons for this is the absence of a fast and efficient model of vascular tissue aging. In this study, ex vivo aortic culture and Matrigel subcutaneous implantation are combined to develop a new model for studying vascular cellular senescence. Eight‐week‐old C57BL/6J mice are used to obtain aortas. Bleomycin is used to induce aortas senescence in vitro. Then, aortas are transplanted to the acceptor mice with Matrigel. Senescence is evaluated using western blotting, quantitative polymerase chain reaction, and senescence‐associated beta‐galactosidase activity. Inflammatory cytokines are detected using Luminex Liquid Suspension Chip. RNA levels are analyzed by transcriptome sequencing. The results revealed that vessels in the bleomycin group exhibited significant senescence than those in the control group that can be enhanced by stripping vessel adventitia. The levels of cytokines such as interleukin (IL‐2, IL‐1β, and IL‐6 increased significantly in the ex vivo model. Furthermore, transcriptome sequencing revealed 56 significantly differentially expressed genes (DEGs) in ex vivo model vessels compared with those in naturally aging aortas. In conclusion, this study introduces a cost‐effective and time‐saving vessel senescence model for vascular cellular senescence.