Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae
Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae
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DOI：
10.1016/j.bbrc.2006.03.117
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发表时间：
2006-05-26
影响因子：
3.1
通讯作者：
Grimmelikhuijzen, CJP
中科院分区：
文献类型：
--
作者：
Belmont, M;Cazzamali, G;Grimmelikhuijzen, CJP
冈比亚按蚊是疟疾的重要传播媒介，疟疾是世界上最严重的人类寄生虫病之一，每年导致多达 270 万人死亡。为了有助于我们对冈比亚蚊子和疟疾传播的了解，我们现在从这种蚊子中克隆了四种与进化相关的G蛋白偶联受体（GPCR），并在中国仓鼠卵巢细胞中表达它们。在筛选了 33 种昆虫或其他无脊椎动物神经肽和 8 种生物胺的文库后，我们可以将其中的 3 种 GPCR 鉴定（去孤儿化）为：脂肪运动激素 (AKH) 受体（EC50 对于冈比亚 A. gambiae AKH，3 x 10(-9) M）、corazonin 受体（EC50 对于 A. gambiae corazonin，4 x 10(-9) M) 和甲壳动物心脏活性肽 (CCAP) 受体（冈比亚 A. 冈比亚 CCAP 的 EC50，1 x 10(-9) M）。第四个 GPCR 仍然是一个孤儿，尽管它与冈比亚 A. gambiae 和其他昆虫 AKH 受体的密切进化关系表明它是 AKH 样肽的受体。这是第一篇发表的关于蚊子中与进化相关的 AKH、corazonin 和 CCAP 受体的报告。 (c) 2006 Elsevier Inc. 保留所有权利。
The mosquito Anopheles gambiae is in important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC50 for A. gambiae AKH, 3 x 10(-9) M), a corazonin receptor (EC50 for A. gambiae corazonin, 4 x 10(-9) M), and a crustacean cardioactive peptide (CCAP) receptor (EC50 for A. gambiae CCAP, 1 x 10(-9) M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes. (c) 2006 Elsevier Inc. All rights reserved.