Molecular Characterization of the Transcription Factors in Susceptible Poplar Infected with Virulent Melampsora larici-populina
Molecular Characterization of the Transcription Factors in Susceptible Poplar Infected with Virulent Melampsora larici-populina
复制标题
感染强毒落叶松杨霉的易感杨树转录因子的分子特征
DOI：
10.3390/ijms20194806
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Zhang, Ruizhi
中科院分区：
文献类型：
--
作者：
Chen, Qiaoli;Wang, Jianan;Zhang, Ruizhi
转录因子在白杨的感病性中起着重要作用。本研究以落叶松-杨栅锈菌E4强毒生理小种与“罗布斯塔”（Populus nigra x P. deltoides）的亲和互作为研究对象，分析了5个抗性相关TF组在不同时间点的转录谱，以探讨TF在“罗布斯塔”（Populus nigra x P. deltoides）与落叶松-杨栅锈菌E4强毒生理小种亲和互作中的作用。药敏试验表明，E4的寄生过程可分为侵染期和生产期两个具有代表性的时期。生物信息学分析表明，在这两个阶段，E4感染诱导'罗布斯塔'的TF网络。虽然一些TF反应迅速，积极，大多数TF没有响应E4，特别是在感染阶段。乙烯，茉莉酸和生长素途径下调，而钙结合蛋白上调。没有发现其他显著改变的植物病原体相关基因，这与在没有免疫应答的情况下的病理过程一致，表明在E4的感染阶段大多数TF缺乏应答与'Robusta'的易感性有关。
Transcription factors (TFs) have been shown to play important roles in determining poplar susceptibility. In this study, the transcript profiles of five resistance-related TF groups at different time points were investigated to study the roles of TFs in the compatible interaction between 'Robusta' (Populus nigra x P. deltoides) and the virulent E4 race of Melampsora larici-populina. The susceptibility test indicated that the parasitic process of E4 could be divided into two representative time periods: the infection phase and the production phase. Bioinformatics analysis showed that in these two phases, E4 infection induced a network of TFs in 'Robusta'. Although some TFs responded rapidly and positively, most TFs did not respond to E4, especially during the infection phase. The ethylene, jasmonic acid, and auxin pathways were downregulated, while a calcium-binding protein was upregulated. No other significantly changed phytohormone-related genes were found, which was consistent with the pathological process in the absence of an immune response, suggesting that the lack of response of most TFs during the infection phase of E4 is related to the susceptibility of 'Robusta'.