AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINS
AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINS
复制标题
51 Cr 释放测定法的扩展用于估计小鼠细胞毒素
作者：
W. Boyle
摘要~(51)Cr释放法检测细胞毒性反应具有许多优点，但迄今仅适用于不受红细胞污染的小鼠细胞类型。提出了一种简单的方法，可以选择性地溶解组织细胞制剂中的污染红细胞，而不损害组织细胞。然后，可以通过51Cr释放细胞毒性试验来标记和测试组织细胞。通过对小鼠脾细胞悬液和出血性腹水肉瘤的成功实验，证明了该方法的有效性。
SUMMARY The 51Cr-release assay for measurement of cytotoxic reactions, with its many advantages, has hitherto been applicable only to mouse cell types which occur free from contamination with erythrocytes. A simple method is presented which can selectively lyse contaminating erythrocytes from tissue cell preparations without damaging the tissue cells. The tissue cells can then be labelled and tested by the 51Cr-release cytotoxicity assay. The usefulness of the method is shown by demonstrating its success with suspensions of mouse spleen cells and with a hemorrhagic ascites sarcoma.