CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum
CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum
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CYP4BN6 和 CYP6BQ11 介导杀虫剂敏感性，其表达受赤拟谷盗中 Latrophilin 的调节
DOI：
10.1002/ps.5384
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发表时间：
2019
影响因子：
4.1
通讯作者：
Li Bin
中科院分区：
文献类型：
--
作者：
Xiong Wenfeng;Gao Shanshan;Mao Jinjuan;Wei Luting;Xie Jia;Liu Juanjuan;Bi Jingxiu;Song Xiaowen;Li Bin
许多昆虫细胞色素P450蛋白（CYPs）参与外源化合物（如植物毒素和杀虫剂）的代谢解毒。红粉甲虫是一种主要的农业害虫，破坏储存的谷物和谷类产品。随着其基因组测序的完成，twoT。确定了番石榴种特异性CYP基因CYP4BN6和cyp6bq11。然而，大多数CYPs的功能是否由tccyp4bn6和tccyp6bq11共享尚不清楚，这两个CYPs的上游调控机制也尚不清楚。结果qrt‐PCR分析显示，attccyp4bn6和tccyp6bq11在蛹后期表达量最高，分别主要在成虫的头部和肠道中表达。此外，这两种CYPs的转录本被敌敌畏和呋喃显著诱导，RNA干扰（RNAi）分别靶向它们，增强了甲虫对这两种杀虫剂的敏感性。有趣的是，据报道与杀虫剂敏感性相关的嗜atrophilin（lph）基因敲低后，杀虫剂处理后tccyp4bn6和tccyp6bq11的表达减少，这表明这两个CYP基因受叶绿素调控参与杀虫剂敏感性inT。castaneum。结论本研究结果揭示了CYP基因与杀虫剂敏感性相关的功能和机制，为害虫的合理和可持续防治提供了新的思路。©2019化学工业协会
BACKGROUNDMany insect cytochrome P450 proteins (CYPs) are involved in the metabolic detoxification of exogenous compounds such as plant toxins and insecticides.Tribolium castaneum, the red flour beetle, is a major agricultural pest that damages stored grains and cereal products. With the completion of the sequencing of its genome, twoT. castaneumspecies‐specific CYP genes,CYP4BN6, andCYP6BQ11, were identified. However, it is unknown whether the functions of most CYPs are shared byTcCYP4BN6andTcCYP6BQ11, and the upstream regulatory mechanism of these two CYPs remains elusive.RESULTSQRT‐PCR analysis indicated thatTcCYP4BN6andTcCYP6BQ11were both most highly expressed at the late pupal stage and were mainly observed in the head and gut, respectively, of adults. Moreover, the transcripts of these two CYPs were significantly induced by dichlorvos and carbofuran, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to these two insecticides. Intriguingly, knockdown of thelatrophilin(lph) gene, which has been reported to be related to the insecticide susceptibility, reduced the expression ofTcCYP4BN6andTcCYP6BQ11after insecticide treatment, suggesting that these two CYP genes are regulated bylphto participate in insecticide susceptibility inT. castaneum.CONCLUSIONThese results shed new light on the function and mechanism of CYP genes associated with insecticide susceptibility and could facilitate research on appropriate and sustainable pest control management. © 2019 Society of Chemical Industry