Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets.
Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets.
复制标题
使用超薄贝塞尔光片的多色 4D 荧光显微镜。
DOI：
10.1038/srep26159
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发表时间：
2016-05-18
影响因子：
4.6
通讯作者：
Loy MM
中科院分区：
文献类型：
--
作者：
Zhao T;Lau SC;Wang Y;Su Y;Wang H;Cheng A;Herrup K;Ip NY;Du S;Loy MM
We demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution.
